Siglec-2/CD22 Antibody (BLCAM/1795)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53239
![Flow Cytometry: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239]](https://resources.bio-techne.com/images/products/Siglec-2-CD22-Antibody-BLCAM-1795-Flow-Cytometry-NBP2-53239-img0004.jpg "Flow Cytometry: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239]")
![Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239]](https://resources.bio-techne.com/images/products/Siglec-2-CD22-Antibody-BLCAM-1795-Western-Blot-NBP2-53239-img0008.jpg "Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # BLCAM/1795
Concentration
0.2 mg/ml
Product Summary for Siglec-2/CD22 Antibody (BLCAM/1795)
Immunogen
Recombinant fragment (around aa52-178) of human Siglec-2/CD22 protein (exact sequence is proprietary) (Uniprot: P20273)
Localization
Cell Surface
Marker
B-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54473)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Siglec-2/CD22 Antibody (BLCAM/1795)
Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239]
Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Western Blot Analysis of Raji cell lysate using Siglec-2/CD22 Antibody (BLCAM/1795).![Immunocytochemistry/ Immunofluorescence: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239]](https://resources.bio-techne.com/images/products/Siglec-2-CD22-Antibody-BLCAM-1795-Immunocytochemistry-Immunofluorescence-NBP2-53239-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239]")
Immunocytochemistry/ Immunofluorescence: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239]
Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Immunofluorescent staining of paraformaldehyde-fixed Ramos cells using Siglec-2/CD22 Antibody (BLCAM/1795) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239]](https://resources.bio-techne.com/images/products/Siglec-2-CD22-Antibody-BLCAM-1795-Immunohistochemistry-Paraffin-NBP2-53239-img0006.jpg "Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239]")
Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239]
Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Formalin-fixed, paraffin-embedded human Spleen stained with Siglec-2/CD22 Antibody (BLCAM/1795).![Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239]](https://resources.bio-techne.com/images/products/Siglec-2-CD22-Antibody-BLCAM-1795-Western-Blot-NBP2-53239-img0001.jpg "Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239]")
![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239]](https://resources.bio-techne.com/images/products/Siglec-2-CD22-Antibody-BLCAM-1795-Immunohistochemistry-Paraffin-NBP2-53239-img0002.jpg "Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239]")
![Protein Array: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239]](https://resources.bio-techne.com/images/products/Siglec-2-CD22-Antibody-BLCAM-1795-Protein-Array-NBP2-53239-img0007.jpg "Protein Array: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239]")
Applications for Siglec-2/CD22 Antibody (BLCAM/1795)
Application
Recommended Usage
ELISA
2-4 ug/ml
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Siglec-2/CD22
Long Name
Sialic Acid Binding Ig-like Lectin 2
Alternate Names
BL-CAM, CD22, Siglec2
Gene Symbol
CD22
UniProt
Additional Siglec-2/CD22 Products
Product Documents for Siglec-2/CD22 Antibody (BLCAM/1795)
Product Specific Notices for Siglec-2/CD22 Antibody (BLCAM/1795)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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