Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1F12

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Siglec-2/CD22 Antibodies »

Product Summary for Siglec-2/CD22 Antibody (OTI1F12) - Azide and BSA Free

Immunogen

Full length human recombinant protein of human CD22(NP_001762) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Siglec-2/CD22 Antibody (OTI1F12) - Azide and BSA Free

Western Blot: Siglec-2/CD22 Antibody (OTI1F12)Azide and BSA Free [NBP2-74186]

Western Blot: Siglec-2/CD22 Antibody (OTI1F12)Azide and BSA Free [NBP2-74186]

Western Blot: Siglec-2/CD22 Antibody (OTI1F12) - Azide and BSA Free [NBP2-74186] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD22.
Immunocytochemistry/ Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) - Azide and BSA Free [NBP2-74186]

Immunocytochemistry/ Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) - Azide and BSA Free [NBP2-74186]

Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (OTI1F12) - Azide and BSA Free [NBP2-74186] - Staining in canine blood cells from a verified customer review.
Western Blot: Siglec-2/CD22 Antibody (OTI1F12)Azide and BSA Free [NBP2-74186]

Western Blot: Siglec-2/CD22 Antibody (OTI1F12)Azide and BSA Free [NBP2-74186]

Western Blot: Siglec-2/CD22 Antibody (OTI1F12) - Azide and BSA Free [NBP2-74186] - Analysis of extracts (35ug) from 9 different cell lines by using CD22 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Applications for Siglec-2/CD22 Antibody (OTI1F12) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:400-4000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Siglec-2/CD22

CD22 is a single-pass type 1 membrane glycoprotein found in the cytoplasm and cell membrane of B-lymphocytes. CD22 has at least two isoforms (alpha and beta) produced by alternate splicing. The protein exists predominantly as a monomer of the beta isoform (95 kDa). It is also found as a heterodimer of the beta and alpha isoforms.

Long Name

Sialic Acid Binding Ig-like Lectin 2

Alternate Names

BL-CAM, CD22, Siglec2

Gene Symbol

CD22

Additional Siglec-2/CD22 Products

Product Documents for Siglec-2/CD22 Antibody (OTI1F12) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Siglec-2/CD22 Antibody (OTI1F12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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