Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # 23M14A10

Format

BSA Free

Concentration

1.0 mg/ml

View all Siglec-H Antibodies »

Product Specifications

Immunogen

SkRSHTkRVERPATHMDQA (aa286-304)

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for Siglec-H Antibody (23M14A10) - BSA Free

Western Blot: Siglec-H Antibody (23M14A10)BSA Free [NBP2-27061]

Western Blot: Siglec-H Antibody (23M14A10)BSA Free [NBP2-27061]

Western Blot: Siglec-H Antibody (23M14A10) [NBP2-27061] - WB analysis of mouse heart lysate using Siglec-H antibody (clone 23M14A10) at 3 ug/ml concentration.
Immunohistochemistry-Paraffin: Siglec-H Antibody (23M14A10) - BSA Free [NBP2-27061]

Immunohistochemistry-Paraffin: Siglec-H Antibody (23M14A10) - BSA Free [NBP2-27061]

Immunohistochemistry-Paraffin: Siglec-H Antibody (23M14A10) [NBP2-27061] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal mouse lung using 5 ug/ml concentration of SiglecH antibody (clone 23M14A10). Alkaline Phosphatase based detection was used for final staining development.
Flow Cytometry: Siglec-H Antibody (23M14A10) - BSA Free [NBP2-27061]

Flow Cytometry: Siglec-H Antibody (23M14A10) - BSA Free [NBP2-27061]

Flow Cytometry: Siglec-H Antibody (23M14A10) [NBP2-27061] - Analysis using the PE conjugate of NBP2-27061. Staining of freshly isolated Balb C mouse splenocytes with B220 (clone RA3-6B2) Alexa Fluor 488 antibody and with Rat IgG2a PE (isotype control, left) or anti-mouse Siglec H PE antibody (Clone: 23M14A10, right).
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Applications for Siglec-H Antibody (23M14A10) - BSA Free

Application
Recommended Usage

Flow Cytometry

1ug/10^6cells~

Immunohistochemistry-Paraffin

5ug/ml

Western Blot

3-5ug/ml~
Application Notes
In IHC-P application, 1M EDTA buffer pH 9.0 was used for antigen retrieval. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Siglec-H

Siglec-H, or sialic acid binding immunoglobulin-like lectin H, is a CD33 related protein expressed specifically by plasmacytoid dendritic cells or pDCs. Antigen-mediated delivery by Siglec-H in pDCs inhibits Th cell proliferation and further antibody responses. This leads to lessened expansion and Th1/Th17 polarization. Constant and low density antigen presentation by Siglec-H is thought to lead to an exhaustive type lessening of the response in CD4+ cells but not tolerance. A number of pathways have been proposed for the Siglec-H induced T cell hyporesponsiveness. Ever decreasing activation cycles in the presence of low level but continuous antigen delivery, as that observed with Siglec-H, have also been demonstrated to impart FoxP3+ Tregs immunosuppressive tolerogen-like effects. Ability to identify and control Siglec-H antigen mediated delivery activities with specific antibody provides a focal point for potential development of inflammation controls.

Long Name

Sialic Acid Binding Ig-like Lectin 7

Alternate Names

SiglecH

Gene Symbol

Siglech

Additional Siglec-H Products

Product Documents for Siglec-H Antibody (23M14A10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Siglec-H Antibody (23M14A10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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