Siglec-H Antibody (440c)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11235

Novus Biologicals, part of Bio-Techne
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NBP3-11235
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Flow Cytometry, Functional, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 440c

Concentration

0.1 mg/ml

View all Siglec-H Antibodies »

Product Summary for Siglec-H Antibody (440c)

Immunogen

The monoclonal antibody 440c reacts with Siglec-H, a cell-surface receptor molecule selectively expressed on murine natural interferon producing cells (IPC), also called mouse plasmacytoid dendritic cells (pDC).

Specificity

The monoclonal antibody 440c exclusively recognizes mouse Siglec-H in all lymphoid organs under both normal and inflammatory conditions.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Siglec-H Antibody (440c)

Immunohistochemistry-Frozen: Siglec-H Antibody (440c) [NBP3-11235]

Applications for Siglec-H Antibody (440c)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Immunohistochemistry and Flow Cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition of IFN-alpha secretion dilutions have to be made according to the amounts of Siglec-H to be inactivated.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Siglec-H

The monoclonal antibody 440c reacts with Siglec-H, a cell-surface receptor molecule selectively expressed on murine natural interferon producing cells (IPC), also called mouse plasmacytoid dendritic cells (pDC). Siglec H is unique among Sialic acid-binding Ig-like lectins (Siglecs) proteins because it associates with the adaptor protein DAP12. DAP12 recognize certain viruses and CpG-DNA through TLR9, resulting in secretion of IFN-alpha, IL-12 and proinflammatory chemokines. Together these cytokines and chemokines recruite and activate NK cells and T cells as well as modulating the antigen presenting function of dendritic cells (DC). IPC themselves also function as antigen presenting cells that expand memory T cells and induce Th1 differentiation. Therefore IPC may provide a first line of host defense against viral infections by activating both innate and adaptive responses in vivo. The monoclonal antibody 440c exclusively recognizes mouse Siglec-H in all lymphoid organs under both normal and inflammatory conditions. Siglec-H is normal present in the T cell zone of lymph nodes and spleen. Incubation of IPC with antibody 440c in vitro or administration of antibody 440c in vivo reduces secretion of IFN-alpha in response to CpG DNA without causing IPC depletion.

Long Name

Sialic Acid Binding Ig-like Lectin 7

Alternate Names

SiglecH

Gene Symbol

Siglech

Additional Siglec-H Products

Product Documents for Siglec-H Antibody (440c)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Siglec-H Antibody (440c)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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