Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Signal Peptide Peptidase Antibodies »

Product Summary for Signal Peptide Peptidase Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 327 to 377 of human Signal Peptide Peptidase Like 1 using the numbering given in entry NP_110416.1 (GeneID 81502).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Signal Peptide Peptidase Antibody

Western Blot: Signal Peptide Peptidase Antibody [NBP2-32276]

Western Blot: Signal Peptide Peptidase Antibody [NBP2-32276]

Western Blot: Signal Peptide Peptidase Antibody [NBP2-32276] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-SPP antibody NBP2-32276 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: Signal Peptide Peptidase Antibody [NBP2-32276]

Immunoprecipitation: Signal Peptide Peptidase Antibody [NBP2-32276]

Immunoprecipitation: Signal Peptide Peptidase Antibody [NBP2-32276] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-SPP antibody NBP2-32276 used for IP at 6 ug per reaction. SPP was also immunoprecipitated by rabbit anti-SPP antibody BL16569. For blotting immunoprecipitated SPP, NBP2-32276 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Signal Peptide Peptidase Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Signal Peptide Peptidase

Signal Peptide Peptidase is encoded by this gene, which localizes to the endoplasmic reticulum, catalyzes intramembrane proteolysis of some signal peptides after they have been cleaved from a preprotein. This activity is required to generate signal sequence-derived human lymphocyte antigen-E epitopes that are recognized by the immune system, and to process hepatitis C virus core protein. The encoded protein is an integral membrane protein with sequence motifs characteristic of the presenilin-type aspartic proteases. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

dJ324O17.1, EC 3.4.23.-, H13hIMP1, histocompatibility (minor) 13, IMP-1, IMP1MSTP086, Intramembrane protease 1, minor histocompatibility antigen 13, minor histocompatibility antigen H13, Presenilin-like protein 3, PSENL3, PSL3IMPAS, Signal peptide peptidase, signal peptide peptidase beta, SPPIMPAS-1

Gene Symbol

HM13

UniProt

NP_110416.1 (Human)

Additional Signal Peptide Peptidase Products

Product Documents for Signal Peptide Peptidase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Signal Peptide Peptidase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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