SIK3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47278
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: QQEYQELFRHMNQGDAGSLAPSLGGQSMTERQALSYQNADSYHHHTSPQHLLQIRAQECVSQASSPTPPHGYAHQPALMHSESMEEDCSCEGAKDGFQD
Reactivity Notes
Rat (80%). Use in Mouse reported in scientific literature (PMID:31350328).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SIK3 Antibody
![Immunocytochemistry/ Immunofluorescence: SIK3 Antibody [NBP2-47278]](https://resources.bio-techne.com/images/products/SIK3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47278-img0006.jpg "Immunocytochemistry/ Immunofluorescence: SIK3 Antibody [NBP2-47278]")
Immunocytochemistry/ Immunofluorescence: SIK3 Antibody [NBP2-47278]
Immunocytochemistry/Immunofluorescence: SIK3 Antibody [NBP2-47278] - Staining of human cell line CACO-2 shows localization to nucleoplasm. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: SIK3 Antibody [NBP2-47278]](https://resources.bio-techne.com/images/products/SIK3-Antibody-Immunohistochemistry-Paraffin-NBP2-47278-img0005.jpg "Immunohistochemistry-Paraffin: SIK3 Antibody [NBP2-47278]")
Immunohistochemistry-Paraffin: SIK3 Antibody [NBP2-47278]
Immunohistochemistry-Paraffin: SIK3 Antibody [NBP2-47278] - Staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts. Leydig cells exhibited strong nuclear staining.Applications for SIK3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
Reported in scientific literature (PMID:31350328)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SIK3
Long Name
Serine/threonine-protein kinase SIK3
Alternate Names
KIAA0999, L19, QSK, SIK-3
Gene Symbol
SIK3
Additional SIK3 Products
Product Documents for SIK3 Antibody
Product Specific Notices for SIK3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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