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SIL1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20364

Novus Biologicals, part of Bio-Techne
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NBP2-20364

Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (95%), Mouse (92%), Primate (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for SIL1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human SIL1. The exact sequence is proprietary.

Localization

Endoplasmic reticulum lumen

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SIL1 Antibody

Western Blot: SIL1 Antibody [NBP2-20364]

Western Blot: SIL1 Antibody [NBP2-20364]

Western Blot: SIL1 Antibody [NBP2-20364] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SIL1 antibody [N1C2] diluted at 1:5000.
Immunohistochemistry-Paraffin: SIL1 Antibody [NBP2-20364]

Immunohistochemistry-Paraffin: SIL1 Antibody [NBP2-20364]

Immunohistochemistry-Paraffin: SIL1 Antibody [NBP2-20364] - human colon carcinoma, using SIL1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: SIL1 Antibody [NBP2-20364]

Western Blot: SIL1 Antibody [NBP2-20364]

Western Blot: SIL1 Antibody [NBP2-20364] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:5000.

Applications for SIL1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SIL1

This gene encodes a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Alternate transcriptional splice variants have been characterized. [provided by RefSeq]

Alternate Names

BAPMarinesco-Sjogren syndrome, BiP-associated protein, MSS, nucleotide exchange factor SIL1, SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae), ULG5

Gene Symbol

SIL1

UniProt

Additional SIL1 Products

Product Documents for SIL1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SIL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.