SIN3A Antibody (10G3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26325
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10G3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for SIN3A Antibody (10G3)
Immunogen
A synthesized peptide derived from Human SIN3A [UniProt Q96ST3]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SIN3A Antibody (10G3)
Immunohistochemistry: SIN3A Antibody (10G3) [NBP3-26325] -
Immunohistochemistry: SIN3A Antibody (10G3) [NBP3-26325] - Image of SIN3A Antibody (10G3) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: SIN3A Antibody (10G3) [NBP3-26325] -
Immunohistochemistry: SIN3A Antibody (10G3) [NBP3-26325] - Image of SIN3A Antibody (10G3) diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: SIN3A Antibody (10G3) [NBP3-26325] -
Western Blot: SIN3A Antibody (10G3) [NBP3-26325] - Positive Western Blot detected in: K562 whole cell lysate, MCF-7 whole cell lysate.All lanes: SIN3A Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 146 kDa
Observed band size: 146 kDa
Applications for SIN3A Antibody (10G3)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: SIN3A
Long Name
SIN3 Homolog A
Alternate Names
DKFZP434K2235, FLJ90319, Histone deacetylase complex subunit Sin3a, KIAA0700, paired amphipathic helix protein Sin3a, SIN3 homolog A, transcription regulator (yeast), SIN3 homolog A, transcriptional regulator (yeast), Transcriptional corepressor Sin3a, transcriptional co-repressor Sin3A, transcriptional regulator, SIN3A
Gene Symbol
SIN3A
Additional SIN3A Products
Product Documents for SIN3A Antibody (10G3)
Product Specific Notices for SIN3A Antibody (10G3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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