SIN3B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20367

Novus Biologicals, part of Bio-Techne
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NBP2-20367
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SIN3B Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SIN3B. The exact sequence is proprietary.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24691445).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SIN3B Antibody

Western Blot: SIN3B Antibody [NBP2-20367]

Western Blot: SIN3B Antibody [NBP2-20367]

Western Blot: SIN3B Antibody [NBP2-20367] - Sample (30 ug of whole cell lysate) A: HCT116 5% SDS PAGE diluted at 1:500
Immunocytochemistry/ Immunofluorescence: SIN3B Antibody [NBP2-20367]

Immunocytochemistry/ Immunofluorescence: SIN3B Antibody [NBP2-20367]

Immunocytochemistry/Immunofluorescence: SIN3B Antibody [NBP2-20367] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SIN3B protein stained by SIN3B antibody [N1N2], N-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.
Immunohistochemistry-Paraffin: SIN3B Antibody [NBP2-20367]

Immunohistochemistry-Paraffin: SIN3B Antibody [NBP2-20367]

Immunohistochemistry-Paraffin: SIN3B Antibody [NBP2-20367] - MCF7 xenograft, using SIN3B antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
View 1 more images

Applications for SIN3B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SIN3B

Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription.

Alternate Names

Histone deacetylase complex subunit Sin3b, KIAA0700paired amphipathic helix protein Sin3b, SIN3 homolog B, transcription regulator (yeast), SIN3 homolog B, transcriptional regulator, SIN3 homolog B, transcriptional regulator (yeast), Transcriptional corepressor Sin3b

Gene Symbol

SIN3B

UniProt

O75182 (Human)

Additional SIN3B Products

Product Documents for SIN3B Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SIN3B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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