SIRP alpha/CD172a Antibody (Hospital for Sick Children patent anti-SIRPA) - Low Endotoxin, Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-28670

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG4 Clone # Hospital for Sick Children patent anti-SIRPA

Format

Low Endotoxin, Azide and BSA Free

Concentration

LYOPH mg/ml

View all SIRP alpha/CD172a Antibodies »

Product Specifications

Immunogen

SIRPa / CD172a

Clonality

Monoclonal

Host

Human

Isotype

IgG4

Endotoxin Level

< 0.001EU/ug,determined by LAL method.

Description

Expressed from CHO. The heavy chain type is huIgG4SP, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Upon receipt, store immediately at -20C or lower for 24 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Scientific Data Images

SIRP alpha/CD172a Antibody (Hospital for Sick Children patent anti-SIRPA)

ELISA: SIRP alpha/CD172a Antibody (Hospital for Sick Children patent anti-SIRPA)[NBP3-28670] -

Immobilized human SIRP alpha V1 FC at 4 ug/mL can bind Hospital for Sick Children patent SIRP alpha/CD172a Antibody (Hospital for Sick Children patent anti-SIRPA), EC50=0.007292 ug/mL.
SIRP alpha/CD172a Antibody (Hospital for Sick Children patent anti-SIRPA)

Flow Cytometry: SIRP alpha/CD172a Antibody (Hospital for Sick Children patent anti-SIRPA)[NBP3-28670] -

Hospital for Sick Children patent SIRP alpha/CD172a Antibody (Hospital for Sick Children patent anti-SIRPA) FACS Blocking was evaluated using human CCRF-CEM cells. The IC50 was approximately 3.289 ug/ml.

Applications

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Format

Low Endotoxin, Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: SIRP alpha/CD172a

Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1), SHPS-1 (2,3), MyD-1 (4), BIT (5,6) and p84 (7). SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2 (1,2,5,8). SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes (1,2,5,8).

Long Name

Signal-regulatory Protein alpha

Alternate Names

BIT, CD172a, MFR, MYD-1, SHPS1, SIRPA

Gene Symbol

SIRPA

UniProt

P78324

Additional SIRP alpha/CD172a Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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