Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Mouse, Rat

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OX-41

Concentration

1 mg/ml

View all SIRP alpha/CD172a Antibodies »

Product Specifications

Immunogen

Rat peritoneal macrophages

Reactivity Notes

Reacts with Rat. Use in Mouse reported in scientific literature (PMID:34413330)

Localization

Membrane; single pass type I membrane protein.

Specificity

NB100-65530 recognizes rat CD172a, also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons. MRC OX-41 binds to a different epitope on SIRP than ED9. It does not block the interaction of CD172a - CD47.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for SIRP alpha/CD172a Antibody (OX-41)

Immunohistochemistry: SIRP alpha/CD172a Antibody (OX-41) - BSA Free [NB100-65530]

Immunohistochemistry: SIRP alpha/CD172a Antibody (OX-41) - BSA Free [NB100-65530]

Immunohistochemistry: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power
Immunohistochemistry: SIRP alpha/CD172a Antibody (OX-41) - BSA Free [NB100-65530]

Immunohistochemistry: SIRP alpha/CD172a Antibody (OX-41) - BSA Free [NB100-65530]

Immunohistochemistry: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Low power
Immunohistochemistry: SIRP alpha/CD172a Antibody (OX-41) - BSA Free [NB100-65530]

Immunohistochemistry: SIRP alpha/CD172a Antibody (OX-41) - BSA Free [NB100-65530]

Immunohistochemistry: SIRP alpha/CD172a Antibody (OX-41) [NB100-65530] - Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Medium powerPrevious
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Applications for SIRP alpha/CD172a Antibody (OX-41)

Application
Recommended Usage

Flow Cytometry

1:10-1:50

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This product requires protein digestion pretreatment of paraffin sections e.g. trypsin or pronase. Use in ICC/IF reported in scientific literature (PMID:34413330).

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SIRP alpha/CD172a

Protein tyrosine phosphatases (PTPases) SHP1 and SHP2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1),SHPS1 , MyD1, BIT and p84. SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP2. SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes.

Long Name

Signal-regulatory Protein alpha

Alternate Names

BIT, CD172a, MFR, MYD-1, SHPS1, SIRPA

Gene Symbol

SIRPA

UniProt

P97710

Additional SIRP alpha/CD172a Products

Product Documents for SIRP alpha/CD172a Antibody (OX-41)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SIRP alpha/CD172a Antibody (OX-41)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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