Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all SIRP alpha/CD172a Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human SIRP alpha 1. The immunogen is located within the last 50 amino acids of SIRP alpha. Amino Acid Squence: KPEPSFSEYASVQVPRK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (88%)

Specificity

Recognizes SIRP alpha 1, 2 and 3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

75-110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SIRP alpha/CD172a Antibody - BSA Free

Western Blot: SIRP alpha/CD172a AntibodyBSA Free [NBP1-77045]

Western Blot: SIRP alpha/CD172a AntibodyBSA Free [NBP1-77045]

Western Blot: SIRP alpha/CD172a Antibody [NBP1-77045] - Analysis of SIRP alpha in THP-1 whole cell lysate with anti-SIRP alpha using this antibody at 1:2000 dilution.
Immunocytochemistry/ Immunofluorescence: SIRP alpha/CD172a Antibody - BSA Free [NBP1-77045]

Immunocytochemistry/ Immunofluorescence: SIRP alpha/CD172a Antibody - BSA Free [NBP1-77045]

Immunocytochemistry/Immunofluorescence: SIRP alpha/CD172a Antibody [NBP1-77045] - THP-1 cells with SIRP alpha antibody at 1 ug/ml.
SIRP alpha/CD172a Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SIRP alpha/CD172a Antibody - BSA Free [NBP1-77045] -

Immunocytochemistry/ Immunofluorescence: SIRP alpha/CD172a Antibody - BSA Free [NBP1-77045] - Immunocytochemistry of SIRP alpha in THP-1 cells with SIRP alpha antibody at 1 u/mL.
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Applications for SIRP alpha/CD172a Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1 ug/ml

Western Blot

0.5-1 ug/ml
Application Notes
75 - 110 kDa bands should be detected in Western Blot.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SIRP alpha/CD172a

Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPalpha, SHPS-1, MyD-1, BIT and p84. SIRPalpha is a new gene family containing at least fifteen members. SIRPalpha is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2. SIRPalpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes.

Long Name

Signal-regulatory Protein alpha

Alternate Names

BIT, CD172a, MFR, MYD-1, SHPS1, SIRPA

Gene Symbol

SIRPA

UniProt

P78324

Additional SIRP alpha/CD172a Products

Product Documents for SIRP alpha/CD172a Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SIRP alpha/CD172a Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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