SIRP alpha/CD172a Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-56723

Novus Biologicals, part of Bio-Techne
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NBP2-56723
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SIRP alpha/CD172a Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: RIRQKKAQGSTSSTRLHEPEKNAREITQDTNDITYADLNLPKGKKPAPQAAEPN

Reactivity Notes

Mouse 81%, Rat 80%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SIRP alpha/CD172a Antibody

Western Blot: SIRP alpha/CD172a Antibody [NBP2-56723]

Western Blot: SIRP alpha/CD172a Antibody [NBP2-56723]

Western Blot: SIRP alpha/CD172a Antibody [NBP2-56723] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.
Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-56723] - Staining of human cerebellum shows moderate cytoplasmic positivity in cells in granular layer, neuropil was distinctly stained.
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Applications for SIRP alpha/CD172a Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SIRP alpha/CD172a

Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1), SHPS-1 (2,3), MyD-1 (4), BIT (5,6) and p84 (7). SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2 (1,2,5,8). SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes (1,2,5,8).

Long Name

Signal-regulatory Protein alpha

Alternate Names

BIT, CD172a, MFR, MYD-1, SHPS1, SIRPA

Gene Symbol

SIRPA

Additional SIRP alpha/CD172a Products

Product Documents for SIRP alpha/CD172a Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SIRP alpha/CD172a Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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