Sirtuin 1/SIRT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49540

Novus Biologicals, part of Bio-Techne
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NBP1-49540
NBP1-49540SS
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Knockout Validated, Simple Western, Western Blot

Cited:

Flow Cytometry, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all Sirtuin 1/SIRT1 Antibodies »

Product Specifications

Immunogen

A genomic peptide made to an internal region of the human SIRT1 protein (within residues 400-550). [Swiss-Prot Q96EB6]

Localization

Nucleus, PML body. Note: Recruited to the nuclear bodies via its interaction with PML

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sirtuin 1/SIRT1 Antibody

Knockout Validated: Sirtuin 1/SIRT1 Antibody [NBP1-49540]

Knockout Validated: Sirtuin 1/SIRT1 Antibody [NBP1-49540]

Knockout Validated: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SIRT1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-49540) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for SIRT1 at approximately 130 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: Sirtuin 1/SIRT1 Antibody [NBP1-49540]

Western Blot: Sirtuin 1/SIRT1 Antibody [NBP1-49540]

Western Blot: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Analysis of SIRT1 in HeLa whole cell lyates.
Immunohistochemistry: Sirtuin 1/SIRT1 Antibody [NBP1-49540]

Immunohistochemistry: Sirtuin 1/SIRT1 Antibody [NBP1-49540]

Immunohistochemistry: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Immunohistochemical analysis of SIRT1 in human renal cell carcinoma.
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Applications for Sirtuin 1/SIRT1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Simple Western

1:100

Western Blot

1:1000
Application Notes
This SIRT1 antibody is useful in Immunocytochemistry/Immunfluorescence, Immunhistochemistry paraffin embedded sections and Western blot. In Western blot a band is seen ~105 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 0.1% BSA, and 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sirtuin 1/SIRT1

SIRT1 (mammalian homologue of silent information regulator 2), is a NAD-dependent class III deacetylase which belongs to mammalian sirtuin family and generates enzymatic activity in the presence of NAD+. SIRT1 is primarily localized in the nucleus and deacetylates not only histones but also a number of other transcription factors/cofactors, such as p53, FOXO (forkhead), PPAR- gamma coactivator-1 alpha, and NFkB, thus affecting the critical cellular pathways involved in stress response. SIRT1 plays an important role in the energy metabolism by interacting with PPAR-gamma and inhibiting its transcriptional activity towards genes mediating fat storage through docking with its cofactors. SIRT1 also plays an essential function in mediating cellular survival by deacetylating substrates such as FOXO, p53 , DNA repair factor Ku70 and NFkB. SIRT1 has been shown to get upregulated in response to stress-induced cell death, especially upon oxidative stress and calorie restriction, and SIRT1 knockout mice fail to extend lifespan induced by calorie restriction. Moreover, expression of SIRT1 gets increased to promote neuronal survival in Alzheimer's disease model as well as in primary neurons subjected to neurotoxic insult.

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2-like protein 1, SIR2alpha, SIR2L1, SIRT1

Entrez Gene IDs

23411 (Human); 93759 (Mouse)

Gene Symbol

SIRT1

UniProt

Q96EB6 (Human), Q923E4 (Mouse)

Additional Sirtuin 1/SIRT1 Products

Product Documents for Sirtuin 1/SIRT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Sirtuin 1/SIRT1 Antibody

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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