Sirtuin 1/SIRT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27205

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-27205
NBP2-27205SS
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Porcine, Amphibian, Bovine, Canine, Chicken, Chinese Hamster, Equine, Feline, Hamster, Primate, Rabbit

Cited:

Human, Mouse, Porcine, Hamster

Predicted:

Avian (100%), Chimpanzee (100%), Monkey (100%), Orangutan (100%), Rat (100%), Reptile (100%). Backed by our 100% Guarantee.

Applications

Validated:

Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Sirtuin 1/SIRT1 Antibodies »

Product Specifications

Immunogen

A portion of amino acids 400-450 from human SIRT1 was used as the immunogen for this antibody.

Reactivity Notes

The amino acid sequence used as immunogen for this antibody is 100% homologous in Frog, and White-cheeked Gibbon, and 89% homologous in Zebrafish. Use in Hamster reported in scientific literature (PMID:24585438).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sirtuin 1/SIRT1 Antibody

Western Blot: Sirtuin 1/SIRT1 Antibody [NBP2-27205]

Western Blot: Sirtuin 1/SIRT1 Antibody [NBP2-27205]

Western Blot: Sirtuin 1/SIRT1 Antibody [NBP2-27205] - Analysis of SIRT1 using SIRT1 antibody. HeLa lysate probed with 1) prebleed, 2) SIRT1 antibody and 3) mouse NIH 3T3 lysate probed with SIRT1 antibody at2 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Simple Western: Sirtuin 1/SIRT1 Antibody [NBP2-27205]

Simple Western: Sirtuin 1/SIRT1 Antibody [NBP2-27205]

Simple Western: Sirtuin 1/SIRT1 Antibody [NBP2-27205] - Simple Western lane view shows a specific band for Sirtuin 1/SIRT1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for Sirtuin 1/SIRT1 Antibody

Application
Recommended Usage

Simple Western

1:100

Western Blot

2-4ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 3.5 using NBP2-27205 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sirtuin 1/SIRT1

SIRT1 is a nuclear protein and member of the sirtuin protein family of deacetylating proteins. SIRT1 is involved in regulating a number of cellular pathways including apoptosis and cell senescence, longevity and aging (1-3). Not surprisingly, SIRT1 targets to forkhead (FOXO) transcription factors. SIRT1 physically binds to the p65/RelA protein in the NF-kB complex and inhibits the transcriptional activity by deacetylating the p65 component (4). SIRT1 anti-inflammatory responses have also been demonstrated using resveratrol, a potent activator of SIRT1, which has been shown to potentiate the SIRT1-mediated anti-inflammatory response (5). Detailed involvement of SIRT1 and NF-kB, and the control and interconnection of the Innate Immune and inflammatory response and aging, is reviewed by Salminen et al (6).

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2-like protein 1, SIR2alpha, SIR2L1, SIRT1

Gene Symbol

SIRT1

Additional Sirtuin 1/SIRT1 Products

Product Documents for Sirtuin 1/SIRT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Sirtuin 1/SIRT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.