Sirtuin 2/SIRT2 Antibody (1E1U3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16301

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16301-100ul
NBP3-16301-20ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1E1U3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Sirtuin 2/SIRT2 Antibodies »

Product Summary for Sirtuin 2/SIRT2 Antibody (1E1U3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Sirtuin 2/SIRT2 (Q8IXJ6). MAEPDPSHPLETQAGKVQEAQDSDSDSEGGAAGGEADMDFLRNLFSQTLSLGSQKERLLDELTLEGVARYMQSERCRRVICLVGAGISTSAGIPDFRSPS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sirtuin 2/SIRT2 Antibody (1E1U3)

Western Blot: Sirtuin 2/SIRT2 Antibody (1E1U3) [NBP3-16301]

Western Blot: Sirtuin 2/SIRT2 Antibody (1E1U3) [NBP3-16301]

Western Blot: Sirtuin 2/SIRT2 Antibody (1E1U3) [NBP3-16301] - Western blot analysis of extracts of various cell lines, using Sirtuin 2/SIRT2 antibody (NBP3-16301) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for Sirtuin 2/SIRT2 Antibody (1E1U3)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Sirtuin 2/SIRT2

Silent information regulator 2 (Sir2) family of enzymes has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging. O-acetyl-ADP-ribose causes a delay/block in oocyte maturation and results in a delay/block in embryo cell division in blastomeres. It has been demonstrated that the production of O-acetyl-ADP-ribose is evolutionarily conserved among Sir2-like enzymes from yeast, Drosophila, and human. Also, endogenous yeast Sir2 complex from telomeres was shown to generate O-acetyl-ADP-ribose (1). SIRT2 is a predominantly cytoplasmic protein that colocalizes with microtubules. SIRT2 deacetylates lysine-40 of alpha-tubulin both in vitro and in vivo. Knockdown of SIRT2 via siRNA results in tubulin hyperacetylation. SIRT2 colocalizes and interacts in vivo with HDAC6, another tubulin deacetylase (2). Cytoskeleton-related protein, SIRT2 is down-regulated in gliomas, and data suggests that ectopic expression of SIRT2 in glioma cell lines led to the perturbation of the microtubule network (3).

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2L2, SIRT2

Gene Symbol

SIRT2

Additional Sirtuin 2/SIRT2 Products

Product Documents for Sirtuin 2/SIRT2 Antibody (1E1U3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Sirtuin 2/SIRT2 Antibody (1E1U3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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