Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08293

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PCRP-SIRT2-1A8

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Sirtuin 2/SIRT2 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Sirtuin 2/SIRT2 protein (Uniprot: Q8IXJ6 )

Localization

Cytoplasm > cytoskeleton. Colocalizes with microtubules

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07358).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free [NBP3-08293]

Immunocytochemistry/ Immunofluorescence: Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free [NBP3-08293]

Immunocytochemistry/Immunofluorescence: Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free [NBP3-08293] - Immunofluorescence Analysis of PFA-fixed U-87 cells using Sirtuin 2/SIRT2 Mouse Monoclonal Antibody (PCRP-SIRT2-1A8) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).
Flow Cytometry: Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free [NBP3-08293]

Flow Cytometry: Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free [NBP3-08293]

Flow Cytometry: Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free [NBP3-08293] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells. Sirtuin 2/SIRT2 Mouse Monoclonal Antibody (PCRP-Sirtuin 2/SIRT2-1A8) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).
Immunocytochemistry/ Immunofluorescence: Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free [NBP3-08293]

Immunocytochemistry/ Immunofluorescence: Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free [NBP3-08293]

Immunocytochemistry/Immunofluorescence: Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free [NBP3-08293] - Immunofluorescence Analysis of PFA-fixed HeLa cells using Sirtuin 2/SIRT2 Mouse Monoclonal Antibody (PCRP-SIRT2-1A8) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).
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Applications for Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Sirtuin 2/SIRT2

Silent information regulator 2 (Sir2) family of enzymes has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging. O-acetyl-ADP-ribose causes a delay/block in oocyte maturation and results in a delay/block in embryo cell division in blastomeres. It has been demonstrated that the production of O-acetyl-ADP-ribose is evolutionarily conserved among Sir2-like enzymes from yeast, Drosophila, and human. Also, endogenous yeast Sir2 complex from telomeres was shown to generate O-acetyl-ADP-ribose (1). SIRT2 is a predominantly cytoplasmic protein that colocalizes with microtubules. SIRT2 deacetylates lysine-40 of alpha-tubulin both in vitro and in vivo. Knockdown of SIRT2 via siRNA results in tubulin hyperacetylation. SIRT2 colocalizes and interacts in vivo with HDAC6, another tubulin deacetylase (2). Cytoskeleton-related protein, SIRT2 is down-regulated in gliomas, and data suggests that ectopic expression of SIRT2 in glioma cell lines led to the perturbation of the microtubule network (3).

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2L2, SIRT2

Gene Symbol

SIRT2

Additional Sirtuin 2/SIRT2 Products

Product Documents for Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Sirtuin 2/SIRT2 Antibody (PCRP-SIRT2-1A8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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