Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Rabbit (100%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Sirtuin 2/SIRT2 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of the human SIRT2. The immunogen is located within the last 50 amino acids of SIRT2. Amino Acid Squence: SPKKSPPPAKDEARTTERE

Marker

Early Oligodendrocyte Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Sirtuin 2/SIRT2 Antibody - BSA Free

Western Blot: Sirtuin 2/SIRT2 AntibodyBSA Free [NBP1-76879]

Western Blot: Sirtuin 2/SIRT2 AntibodyBSA Free [NBP1-76879]

Western Blot: Sirtuin 2/SIRT2 Antibody [NBP1-76879] - Mouse brain lysate with SIRT2 antibody at (A) 1 and (B) 2 ug/mL.
Sirtuin 2/SIRT2 Antibody - BSA Free

Immunohistochemistry: Sirtuin 2/SIRT2 Antibody - BSA Free [NBP1-76879] -

Immunohistochemistry: Sirtuin 2/SIRT2 Antibody - BSA Free [NBP1-76879] - Immunohistochemical staining of Human Brain tissue using Sirtuin 2/SIRT2 antibody at 2.5 u/mL.
Sirtuin 2/SIRT2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Sirtuin 2/SIRT2 Antibody - BSA Free [NBP1-76879] -

Immunocytochemistry/ Immunofluorescence: Sirtuin 2/SIRT2 Antibody - BSA Free [NBP1-76879] - Immunofluorescence of Sirtuin 2/SIRT2 in Human Brain tissue with Sirtuin 2/SIRT2 antibody at 5 ug/mL.

Applications for Sirtuin 2/SIRT2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5 ug/mL

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1-2 ug/ml

Reviewed Applications

Read 1 review rated 5 using NBP1-76879 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sirtuin 2/SIRT2

The founding member of the sirtuin protein family was the silent information regulator 2 protein (Sir2p) of Saccharomyces cervisiae, an NAD+-dependent histone deacetylase (HDAC) that regulates chromatin silencing. The SIR2 family of genes are highly conserved from prokaryotes to eukaryotes. Mammals have seven homologs of Sir2p, SIRT1-7, which are involved in diverse processes ranging from transcriptional regulation, cell cycle progression and DNA-damage repair to aging. SIRT2 is a predominantly cytoplasmic protein that colocalizes with microtubules and can deacetylate alpha-tubulin and regulate progression through the cell cycle. Most Sirtuins are highly expressed in brain and testis, while Sirt2 expression is higher in fetal relative to adult brain. Recent studies on SIRT2 support the therapeutic utility of inhibitors for the treatment of neurodegenerative diseases such as Parkinson's disease.

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2L2, SIRT2

Entrez Gene IDs

22933 (Human)

Gene Symbol

SIRT2

UniProt

Q8IXJ6

Additional Sirtuin 2/SIRT2 Products

Product Documents for Sirtuin 2/SIRT2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Sirtuin 2/SIRT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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