Sirtuin 3/SIRT3 Antibody (6B2A1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52563

Novus Biologicals, part of Bio-Techne
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NBP2-52563
NBP2-52563-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6B2A1

Format

BSA Free

Concentration

1 mg/ml

View all Sirtuin 3/SIRT3 Antibodies »

Product Summary for Sirtuin 3/SIRT3 Antibody (6B2A1) - BSA Free

Immunogen

Purified recombinant fragment of human Sirtuin 3/SIRT3 (AA: 155-290) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Sirtuin 3/SIRT3 Antibody (6B2A1) - BSA Free

Western Blot: Sirtuin 3/SIRT3 Antibody (6B2A1)BSA Free [NBP2-52563]

Western Blot: Sirtuin 3/SIRT3 Antibody (6B2A1)BSA Free [NBP2-52563]

Western Blot: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Analysis using SIRT3 mouse mAb against PANC-1 (1) cell lysate.
Immunohistochemistry Whole-Mount: Sirtuin 3/SIRT3 Antibody (6B2A1) - BSA Free [NBP2-52563]

Immunohistochemistry Whole-Mount: Sirtuin 3/SIRT3 Antibody (6B2A1) - BSA Free [NBP2-52563]

Immunohistochemistry Whole-Mount: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Cochlear whole mount from wild-type and Sirt3-KO mice, immunolabeled with MYO7, SIRT3, and DAPI. The cross-hairs show the position from which the side view is taken. SIRT3 immunoreactivity only is shown in the right panels. IHC-WhMt image submitted by a verified customer review.
Flow Cytometry: Sirtuin 3/SIRT3 Antibody (6B2A1) - BSA Free [NBP2-52563]

Flow Cytometry: Sirtuin 3/SIRT3 Antibody (6B2A1) - BSA Free [NBP2-52563]

Flow Cytometry: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Analysis of HeLa cells using SIRT3 mouse mAb (green) and negative control (red).
View 3 more images

Applications for Sirtuin 3/SIRT3 Antibody (6B2A1) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry

1:200 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready. This Sirtuin 3/SIRT3 Antibody (6B2A1) is validated for IHC-WhMt from a verified customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-52563 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sirtuin 3/SIRT3

SIRT3 is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).

Long Name

Silent Mating Type Information Regulation 3 Homolog

Alternate Names

SIR2-like protein 3, SIR2L3, SIRT3

Gene Symbol

SIRT3

Additional Sirtuin 3/SIRT3 Products

Product Documents for Sirtuin 3/SIRT3 Antibody (6B2A1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Sirtuin 3/SIRT3 Antibody (6B2A1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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