Sirtuin 3/SIRT3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31029

Novus Biologicals, part of Bio-Techne
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NBP1-31029
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Knockout Validated, Western Blot

Cited:

IF/IHC, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Sirtuin 3/SIRT3 Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Sirtuin 3/SIRT3. The exact sequence is proprietary.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:31931835).

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Sirtuin 3/SIRT3 Antibody

Western Blot: Sirtuin 3/SIRT3 Antibody [NBP1-31029]

Western Blot: Sirtuin 3/SIRT3 Antibody [NBP1-31029]

Western Blot: Sirtuin 3/SIRT3 Antibody [NBP1-31029] - Wild-type (WT) and SIRT3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SIRT3 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Sirtuin 3/SIRT3 Antibody [NBP1-31029]

Immunocytochemistry/ Immunofluorescence: Sirtuin 3/SIRT3 Antibody [NBP1-31029]

Immunocytochemistry/Immunofluorescence: Sirtuin 3/SIRT3 Antibody [NBP1-31029] - Analysis of methanol-fixed A549, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP1-31029]

Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP1-31029]

Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP1-31029] - Cal27 xenograft, using SIRT3 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Applications for Sirtuin 3/SIRT3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
Use in Knockdown Validated reported in scientific literature (PMID:31931835).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sirtuin 3/SIRT3

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.

Long Name

Silent Mating Type Information Regulation 3 Homolog

Alternate Names

SIR2-like protein 3, SIR2L3, SIRT3

Gene Symbol

SIRT3

Additional Sirtuin 3/SIRT3 Products

Product Documents for Sirtuin 3/SIRT3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Sirtuin 3/SIRT3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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