Sirtuin 3/SIRT3 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-02984

Novus Biologicals, part of Bio-Techne
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NBP3-02984-20ul
NBP3-02984-100ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Sirtuin 3/SIRT3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 299-399 of human Sirtuin 3/SIRT3 (NP_036371.1). PQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Sirtuin 3/SIRT3 Antibody - Azide and BSA Free

Knockout Validated: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [NBP3-02984]

Western Blot: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [NBP3-02984]

Western Blot: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Analysis of extracts from wild type(WT) and SIRT3 knockout (KO) 293T cells, using SIRT3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: Sirtuin 3/SIRT3 AntibodyAzide and BSA Free [NBP3-02984]

Western Blot: Sirtuin 3/SIRT3 AntibodyAzide and BSA Free [NBP3-02984]

Western Blot: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Analysis of extracts of various cell lines, using SIRT3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.
Sirtuin 3/SIRT3 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [Sirtuin 3/SIRT3] -

Immunocytochemistry/ Immunofluorescence: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [Sirtuin 3/SIRT3] - Immunofluorescence analysis of NIH/3T3 cells using Sirtuin 3/SIRT3 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
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Applications for Sirtuin 3/SIRT3 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:20 - 1:50

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Sirtuin 3/SIRT3

SIRT3 is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).

Long Name

Silent Mating Type Information Regulation 3 Homolog

Alternate Names

SIR2-like protein 3, SIR2L3, SIRT3

Gene Symbol

SIRT3

Additional Sirtuin 3/SIRT3 Products

Product Documents for Sirtuin 3/SIRT3 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Sirtuin 3/SIRT3 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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