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Sirtuin 5/SIRT5 Antibody (9H12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26323

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-26323-100ul
NBP3-26323-50ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9H12

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from Human Sirtuin 5/SIRT5 [UniProt Q9NXA8]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sirtuin 5/SIRT5 Antibody (9H12)

Sirtuin 5/SIRT5 Antibody (9H12)

Immunohistochemistry: Sirtuin 5/SIRT5 Antibody (9H12) [NBP3-26323] -

Immunohistochemistry: Sirtuin 5/SIRT5 Antibody (9H12) [NBP3-26323] - Image of Sirtuin 5/SIRT5 Antibody (9H12) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for Sirtuin 5/SIRT5 Antibody (9H12)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Sirtuin 5/SIRT5

SIRT5 encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in two transcript variants.

Long Name

Silent Mating Type Information Regulation 5 Homolog

Alternate Names

SIR2L5, SIRT5

Gene Symbol

SIRT5

Additional Sirtuin 5/SIRT5 Products

Product Documents for Sirtuin 5/SIRT5 Antibody (9H12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Sirtuin 5/SIRT5 Antibody (9H12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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