Sirtuin 5/SIRT5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56372

Novus Biologicals, part of Bio-Techne
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NB100-56372
NB100-56372SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Sirtuin 5/SIRT5 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 30-46 of human SIRT5 was used as immunogen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sirtuin 5/SIRT5 Antibody

Western Blot: Sirtuin 5/SIRT5 Antibody [NB100-56372]

Western Blot: Sirtuin 5/SIRT5 Antibody [NB100-56372]

Western Blot: Sirtuin 5/SIRT5 Antibody [NB100-56372] - Immunoblots reveal the levels of sirtuins (SIRT4-7) in the OSCC cell lines HSC-3, UM-SCC-1, and UM-SCC-17B and in normal human oral keratinocytes (K). B-Actin served as loading control. Antibodies include SIRT4 (NB100-1406), SIRT5 (NB100-56372), and SIRT7 (NB100-56364). Image collected and cropped by CiteAb from the following publication (doi.org/10.1002/cncr.25676) licensed under a CC-BY license.
Western Blot: Sirtuin 5/SIRT5 Antibody [NB100-56372]

Western Blot: Sirtuin 5/SIRT5 Antibody [NB100-56372]

Western Blot: Sirtuin 5/SIRT5 Antibody [NB100-56372] - Analysis of Sirtuin 5/SIRT5 in A) human, B) mouse and C) rat intestine cell lysate using this antibody at 2 ug/ml.

Applications for Sirtuin 5/SIRT5 Antibody

Application
Recommended Usage

Western Blot

1-3 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sirtuin 5/SIRT5

SIRT5 is a human member of a family of proteins called Sirtuins (Sir2-like proteins) and are present in prokaryotes and eukaryotes. All Sir2-like proteins have a sirtuin core domain, which contains a series of sequence motifs conserved in organisms ranging from bacteria to humans. Bacterial, yeast and mammalian sirtuins are able to metabolize NAD and possibly at as mono-ADP-ribosyltransferases. The enzymatic function of sirtuins is not yet completely understood but recent reports of histone-activated Sir2-mediated NAD metabolism and NAD-activated Sir2-mediated histone deacetylation suggest a possible coupled reciprocal activation mechanism involving interactions of Sir2 with NAD and the N epsilon-acetyl-lysine groups of acetylated histones.

Long Name

Silent Mating Type Information Regulation 5 Homolog

Alternate Names

SIR2L5, SIRT5

Entrez Gene IDs

68346 (Mouse); 306840 (Rat)

Gene Symbol

SIRT5

UniProt

Q9NXA8

Additional Sirtuin 5/SIRT5 Products

Product Documents for Sirtuin 5/SIRT5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Sirtuin 5/SIRT5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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