SIX1 Antibody (CL0185) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43894

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0185

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SIX1 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q15475, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SIX1 Antibody (CL0185) - Azide and BSA Free

SIX1 Antibody (CL0185) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: SIX1 Antibody [NBP3-43894]

Immunocytochemistry/ Immunofluorescence: SIX1 Antibody [NBP3-43894]

Staining in RH30 cell line with Anti-SIX1 monoclonal antibody) .
SIX1 Antibody (CL0185) - Azide and BSA Free Immunohistochemistry-Paraffin: SIX1 Antibody [NBP3-43894]

Immunohistochemistry-Paraffin: SIX1 Antibody [NBP3-43894]

Analysis in human skeletal muscle and liver tissues using NBP3-43894 antibody. Corresponding SIX1 RNA-seq data are presented for the same tissues.
SIX1 Antibody (CL0185) - Azide and BSA Free Immunohistochemistry-Paraffin: SIX1 Antibody [NBP3-43894]

Immunohistochemistry-Paraffin: SIX1 Antibody [NBP3-43894]

Staining of human endocervix shows strong nuclear positivity in glandular cells.
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Applications for SIX1 Antibody (CL0185) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SIX1

The protein encoded by the SIX1 gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in limb development. Defects in this gene are a cause of autosomal dominant deafness type 23 (DFNA23) and branchiootic syndrome type 3 (BOS3). (provided by RefSeq)

Alternate Names

BOS3, deafness, autosomal dominant 23, DFNA23, homeobox protein SIX1, sine oculis homeobox (Drosophila) homolog 1, Sine oculis homeobox homolog 1, sine oculis homeobox homolog 1 (Drosophila), SIX homeobox 1, TIP39

Gene Symbol

SIX1

Additional SIX1 Products

Product Documents for SIX1 Antibody (CL0185) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SIX1 Antibody (CL0185) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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