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SIX6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82478

Novus Biologicals, part of Bio-Techne
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NBP1-82478
NBP1-82478-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YQEAEKLRGRPLGPVDKYRVRKKFPLPRTIWDGEQKTHCFKERTRNLLREWYLQDPYPNPSKKRELAQATGLTPTQVGNWFKNRRQRDRAAAAKNRLQQQVLSQGSGRALRAEGDGTPEVLGVATSPAASLSSKAATSAISITSSDSECDI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SIX6 Antibody

Western Blot: SIX6 Antibody [NBP1-82478]

Western Blot: SIX6 Antibody [NBP1-82478]

Western Blot: SIX6 Antibody [NBP1-82478] - Analysis in human eye tissue.
Immunocytochemistry/ Immunofluorescence: SIX6 Antibody [NBP1-82478]

Immunocytochemistry/ Immunofluorescence: SIX6 Antibody [NBP1-82478]

Immunocytochemistry/Immunofluorescence: SIX6 Antibody [NBP1-82478] - Staining of human cell line SH-SY5Y shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SIX6 Antibody [NBP1-82478]

Immunohistochemistry-Paraffin: SIX6 Antibody [NBP1-82478]

Immunohistochemistry-Paraffin: SIX6 Antibody [NBP1-82478] - Staining of human pituitary gland shows moderate nuclear positivity.

Applications for SIX6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SIX6

The protein encoded by the Homeobox protein SIX6 gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in eye development. Defects in this gene are a cause of isolated microphthalmia with cataract type 2 (MCOPCT2). (provided by RefSeq)

Alternate Names

homeobox protein SIX6, Homeodomain protein OPTX2, MCOPCT2, Optic homeobox 2, OPTX2, sine oculis homeobox (Drosophila) homolog 6, Sine oculis homeobox homolog 6, sine oculis homeobox homolog 6 (Drosophila), SIX homeobox 6, Six9

Gene Symbol

SIX6

Additional SIX6 Products

Product Documents for SIX6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SIX6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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