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SKA1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92391

Novus Biologicals, part of Bio-Techne
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NBP1-92391

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ELLNKLELEIQYQEQTNNSLKELCESLEEDYKDIEHLKENIPSHLPQVTVTQSCVKGSDLDPEEPIKVEEPEPVKKPPKEQRSIKEM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SKA1 Antibody

Immunocytochemistry/ Immunofluorescence: SKA1 Antibody [NBP1-92391]

Immunocytochemistry/ Immunofluorescence: SKA1 Antibody [NBP1-92391]

Immunocytochemistry/Immunofluorescence: SKA1 Antibody [NBP1-92391] - Staining of human cell line U-2 OS shows positivity in cytoskeleton (microtubules). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SKA1 Antibody [NBP1-92391]

Immunohistochemistry-Paraffin: SKA1 Antibody [NBP1-92391]

Immunohistochemistry-Paraffin: SKA1 Antibody [NBP1-92391] - Staining of human placenta shows weak cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: SKA1 Antibody [NBP1-92391]

Immunohistochemistry-Paraffin: SKA1 Antibody [NBP1-92391]

Immunohistochemistry-Paraffin: SKA1 Antibody [NBP1-92391] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Applications for SKA1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SKA1

SKA1 is a component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essentialfor proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolarattachment on spindle microtubu

Alternate Names

C18orf24, chromosome 18 open reading frame 24, MGC10200, spindle and kinetochore associated complex subunit 1, spindle and kinetochore-associated protein 1, spindle and KT (kinetochore) associated 1

Gene Symbol

SKA1

Additional SKA1 Products

Product Documents for SKA1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SKA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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