SKA3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76311

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all SKA3 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human SKA3. The immunogen is located within amino acids 290 - 340 of SKA3. Amino Acid Squence: STNYPLSKTNSSS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SKA3 Antibody - BSA Free

Western Blot: SKA3 AntibodyBSA Free [NBP1-76311]

Western Blot: SKA3 AntibodyBSA Free [NBP1-76311]

Western Blot: SKA3 Antibody [NBP1-76311] - Human testis tissue lysate with SKA3 antibody at (A) 0.5 and (B) 1 ug/mL.

Applications for SKA3 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

0.5-1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SKA3

Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Like with SKA1 or SKA2, depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged a metaphase-like state. These SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, suggesting the SKA complex plays a key role in spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis.

Alternate Names

spindle and kinetochore associated complex subunit 3, spindle and kinetochore-associated protein 3

Gene Symbol

SKA3

UniProt

NP_659498 (Human)

Additional SKA3 Products

Product Documents for SKA3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SKA3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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