Skp1 Antibody (10G5X5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16734
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10G5X5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 64-163 of human SKP1 (P63208). HHKDDPPPPEDDENKEKRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTCKTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVRKENQWCEEK
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Skp1 Antibody (10G5X5)
Western Blot: Skp1 Antibody (10G5X5) [NBP3-16734]
Western Blot: Skp1 Antibody (10G5X5) [NBP3-16734] - Western blot analysis of extracts of various cell lines, using Skp1 Rabbit mAb (NBP3-16734) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.Immunocytochemistry/ Immunofluorescence: Skp1 Antibody (10G5X5) [NBP3-16734]
Immunocytochemistry/Immunofluorescence: Skp1 Antibody (10G5X5) [NBP3-16734] - Immunofluorescence analysis of NIH-3T3 cells using Skp1 Rabbit mAb (NBP3-16734) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for Skp1 Antibody (10G5X5)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SKP1
Long Name
S-phase Kinase Associated Protein 1
Alternate Names
OCP2, p19a, p19Skp1, SKP1A
Gene Symbol
SKP1
Additional SKP1 Products
Product Documents for Skp1 Antibody (10G5X5)
Product Specific Notices for Skp1 Antibody (10G5X5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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