Skp1 Antibody (1H8)

Novus Biologicals, part of Bio-Techne | Catalog # H00006500-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Bovine

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1H8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SKP1 Antibodies »

Product Specifications

Immunogen

SKP1A (NP_008861, 53 a.a. ~ 160 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AILKKVIQWCTHHKDDPPPPEDDENKEKRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTCKTVANMIKGKTPEEIRKTFNIKNDFTEEEEAQVGSTQFCL

Specificity

SKP1A - S-phase kinase-associated protein 1A (p19A)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Skp1 Antibody (1H8)

Western Blot: Skp1 Antibody (1H8) [H00006500-M01]

Western Blot: Skp1 Antibody (1H8) [H00006500-M01]

Western Blot: Skp1 Antibody (1H8) [H00006500-M01] - SKP1A monoclonal antibody (M01), clone 1H8 Analysis of SKP1A expression in HeLa.
Immunocytochemistry/ Immunofluorescence: Skp1 Antibody (1H8) [H00006500-M01]

Immunocytochemistry/ Immunofluorescence: Skp1 Antibody (1H8) [H00006500-M01]

Immunocytochemistry/Immunofluorescence: Skp1 Antibody (1H8) [H00006500-M01] - Analysis of monoclonal antibody to SKP1A on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Skp1 Antibody (1H8) [H00006500-M01]

Western Blot: Skp1 Antibody (1H8) [H00006500-M01]

Western Blot: Skp1 Antibody (1H8) [H00006500-M01] - Analysis of SKP1 expression in transfected 293T cell line by SKP1A monoclonal antibody (M01), clone 1H8.Lane 1: SKP1 transfected lysate(18.1 KDa).Lane 2: Non-transfected lysate.

Applications for Skp1 Antibody (1H8)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SKP1

This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.

Long Name

S-phase Kinase Associated Protein 1

Alternate Names

OCP2, p19a, p19Skp1, SKP1A

Entrez Gene IDs

6500 (Human)

Gene Symbol

SKP1

OMIM

601434 (Human)

UniProt

NP_008861 (Human)

Additional SKP1 Products

Product Documents for Skp1 Antibody (1H8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Skp1 Antibody (1H8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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