Skp2 Antibody (3C11)

Immunocytochemistry/ Immunofluorescence: Skp2 Antibody (3C11) [NBP3-15197]

Immunocytochemistry/Immunofluorescence: Skp2 Antibody (3C11) [NBP3-15197] - Immunofluorescence staining of HepG2 Cells with Skp2 antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: Skp2 Antibody (3C11) [NBP3-15197]

Flow Cytometry: Skp2 Antibody (3C11) [NBP3-15197] - Overlay histogram showing Hela cells stained with Skp2 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.