Skp2 Antibody (6D4)

Immunocytochemistry/ Immunofluorescence: Skp2 Antibody (6D4) [NBP3-15203]

Immunocytochemistry/Immunofluorescence: Skp2 Antibody (6D4) [NBP3-15203] - Immunofluorescence staining of Hela Cells with at Skp2 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry: Skp2 Antibody (6D4) [NBP3-15203]

Immunohistochemistry: Skp2 Antibody (6D4) [NBP3-15203] - IHC image of Skp2 antibody diluted at 1:100 and staining in paraffin-embedded human placenta tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.