SLC12A3 [p Thr53] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60775
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Rat
Predicted:
Guinea Pig (100%), Hamster (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 conjugated to KLH. Accession # P59158
Modification
p Thr53
Specificity
Specific for endogenous levels of the ~160 kDa SLC12A3 protein phosphorylated at Thr53. Band of interest smearing likely due to glycosylation. Immunolabeling is completely eliminated by treatment with l-phosphatase.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
160 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SLC12A3 [p Thr53] Antibody
![Western Blot: SLC12A3 [p Thr53] Antibody [NBP2-60775]](https://resources.bio-techne.com/images/products/SLC12A3-[p-Thr53]-Antibody-Western-Blot-NBP2-60775-img0001.jpg "Western Blot: SLC12A3 [p Thr53] Antibody [NBP2-60775]")
Western Blot: SLC12A3 [p Thr53] Antibody [NBP2-60775]
Western Blot: SLC12A3 [p Thr53] Antibody [NBP2-60775] - Western blot of mouse kidney lysate showing specificimmunolabeling of the ~160 kDa NCC protein phosphorylatedat Thr53 in the first lane (-). Phosphospecificity is shown in thesecond lane (+) where immunolabeling is completelyeliminated by blot treatment with lambda phosphatase (Lambda-Ptase, 1200 units for 30 min)Applications for SLC12A3 [p Thr53] Antibody
Application
Recommended Usage
Immunohistochemistry
1:100 - 1:10000
Western Blot
1:100-1:2000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SLC12A3
Long Name
Solute Carrier Family 12 (Sodium/Chloride Transporters) Member 3
Alternate Names
NCC, NCCT, TSC
Gene Symbol
SLC12A3
Additional SLC12A3 Products
Product Documents for SLC12A3 [p Thr53] Antibody
Product Specific Notices for SLC12A3 [p Thr53] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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