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SLC25A47 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-57843

Novus Biologicals, part of Bio-Techne
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NB100-57843

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-RYGNPDAKPTKAD corresponding to internal region according to NP_997000.2.

Epitope

RYGNPDAKPTKAD

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SLC25A47 Antibody

Immunohistochemistry-Paraffin: SLC25A47 Antibody [NB100-57843]

Immunohistochemistry-Paraffin: SLC25A47 Antibody [NB100-57843]

Immunohistochemistry-Paraffin: SLC25A47 Antibody [NB100-57843] - IHC analysis of SLC25A47 in paraffin-embedded Human Liver using this antibody at 2.5 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for SLC25A47 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Peptide ELISA

Detection limit 1:16000
Application Notes
WB: Preliminary experiments gave an approx. 45 kDa band in human liver lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.4 kDa band according to NP_997000.2. The 45 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human liver shows staining of nucleoli in select hepatocytes.

Reviewed Applications

Read 1 review rated 3 using NB100-57843 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SLC25A47

C14orf68 is an uncoupling protein which may catalyze the physiological 'proton leak' in liver. Over expression induces the dissipation of mitochondrial membrane potential.

Alternate Names

C14orf68hepatocellular carcinoma-downregulated mitochondrial carrier protein, chromosome 14 open reading frame 68, HDMCPsolute carrier family 25 member 47, Hepatocellular carcinoma down-regulated mitochondrial carrier protein, solute carrier family 25, member 47

Entrez Gene IDs

283600 (Human)

Gene Symbol

SLC25A47

Additional SLC25A47 Products

Product Documents for SLC25A47 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SLC25A47 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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