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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # FP5

Concentration

1 mg/ml

Product Specifications

Immunogen

Mannose binding protein hNIS fusion (AA468-643)

Localization

Membrane

Specificity

Detects approx 97kDa, non-glycosylated version at 68kDa. Other minor bands associated with hNIS at 160kDa, and degradation products at approx 30 kDa, and approx 15kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for SLC5A5/Sodium Iodide Symporter Antibody (FP5)

Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]

Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]

Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Western Blot analysis of Human thyroid lysate showing detection of SLC5A5/Sodium Iodide Symporter protein using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP1-70342). Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP1-70342) at 1:1000.
Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]

Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]

Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP1-70342). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP1-70342) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.
Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]

Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]

Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Effects of 10 uM resveratrol on NIS protein expression. FRTL-5 cells were grown to 60% confluency in 6H5% medium, and then shifted to 5H5% medium for 6 days, before being cultured again in 6H5% medium for 24 h, and finally treated with resveratrol for 48 h. A representative Western blot is shown (top), with quantification (bottom). Data are the normalized means +/-SD (against actin) from three independent experiments (control vehicle: 100%). Control, cells treated with the control vehicle (0.5% ethanol); Resv, cells treated with 10 uM resveratrol. *, p<0.05 versus relevant control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0107936), licensed under a CC-BY license.

Applications for SLC5A5/Sodium Iodide Symporter Antibody (FP5)

Application
Recommended Usage

Immunohistochemistry

1:1000

Western Blot

1:1000
Application Notes
1 ug/ml of Sodium-Iodide Symporter Antibody was sufficient for detection of hNIS in 20 ug of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SLC5A5

The sodium iodide symporter (NIS) is an ion pump that actively transports iodide across the basolateral membrane into thyroid epithelial cells (1, 2). This is important step in the process of iodide organificaton and the formation of triiodothyronine and thyroxine (3).

Long Name

Solute Carrier Family 5 (Sodium Iodide Symporter), Member 5

Alternate Names

NIS, TDH1

Entrez Gene IDs

65199 (Rat)

Gene Symbol

SLC5A5

UniProt

Additional SLC5A5 Products

Product Documents for SLC5A5/Sodium Iodide Symporter Antibody (FP5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SLC5A5/Sodium Iodide Symporter Antibody (FP5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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