SLC5A5/Sodium Iodide Symporter Antibody (FP5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-70342
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # FP5
Concentration
1 mg/ml
Product Specifications
Immunogen
Mannose binding protein hNIS fusion (AA468-643)
Localization
Membrane
Specificity
Detects approx 97kDa, non-glycosylated version at 68kDa. Other minor bands associated with hNIS at 160kDa, and degradation products at approx 30 kDa, and approx 15kDa.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for SLC5A5/Sodium Iodide Symporter Antibody (FP5)
Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]
Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Western Blot analysis of Human thyroid lysate showing detection of SLC5A5/Sodium Iodide Symporter protein using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP1-70342). Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP1-70342) at 1:1000.Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]
Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP1-70342). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP1-70342) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]
Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Effects of 10 uM resveratrol on NIS protein expression. FRTL-5 cells were grown to 60% confluency in 6H5% medium, and then shifted to 5H5% medium for 6 days, before being cultured again in 6H5% medium for 24 h, and finally treated with resveratrol for 48 h. A representative Western blot is shown (top), with quantification (bottom). Data are the normalized means +/-SD (against actin) from three independent experiments (control vehicle: 100%). Control, cells treated with the control vehicle (0.5% ethanol); Resv, cells treated with 10 uM resveratrol. *, p<0.05 versus relevant control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0107936), licensed under a CC-BY license.Applications for SLC5A5/Sodium Iodide Symporter Antibody (FP5)
Application
Recommended Usage
Immunohistochemistry
1:1000
Western Blot
1:1000
Application Notes
1 ug/ml of Sodium-Iodide Symporter Antibody was sufficient for detection of hNIS in 20 ug of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SLC5A5
Long Name
Solute Carrier Family 5 (Sodium Iodide Symporter), Member 5
Alternate Names
NIS, TDH1
Entrez Gene IDs
65199 (Rat)
Gene Symbol
SLC5A5
UniProt
Additional SLC5A5 Products
Product Documents for SLC5A5/Sodium Iodide Symporter Antibody (FP5)
Product Specific Notices for SLC5A5/Sodium Iodide Symporter Antibody (FP5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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