SLC6A3/DAT1 Antibody (mAb16)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22164
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse, Rat, Human (Negative)
Cited:
Mouse, Rat
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # mAb16
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Synthetic peptide corresponding to the N-terminus of rat DAT1. [UniProt# P23977]
Epitope
N-terminus
Reactivity Notes
Little to no human reactivity has been observed.
Localization
Membrane; Multi-pass membrane protein.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for SLC6A3/DAT1 Antibody (mAb16)
Western Blot: SLC6A3/DAT1 Antibody (mAb16) [NBP2-22164]
Western Blot: SLC6A3/DAT1 Antibody (mAb16) [NBP2-22164] - Western blot analysis of DAT protein. Band shown at approx. 75 kDa. Image submitted by verified customer review.Immunocytochemistry/ Immunofluorescence: SLC6A3/DAT1 Antibody (mAb16) [NBP2-22164]
Immunocytochemistry/Immunofluorescence: DAT1 Antibody (mAb16) [NBP2-22164] - The DAT1 antibody was tested in PC12 cells at a 1:250 dilution against DyLight 488 (Green). Actin and nuclei were counterstained with Phalloidin-AlexaFluor 568 (Red) and DAPI (Blue), respectively.Immunohistochemistry-Paraffin: SLC6A3/DAT1 Antibody (mAb16) [NBP2-22164]
Immunohistochemistry-Paraffin: DAT1 Antibody (mAb16) [NBP2-22164] - IHC analysis of DAT1 in mouse brain using DAB with hematoxylin counterstain.Applications for SLC6A3/DAT1 Antibody (mAb16)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:250
Immunohistochemistry
1:100
Immunohistochemistry-Paraffin
1:100
Immunoprecipitation
reported in scientific literature (PMID 20643191)
Western Blot
1:1000
Application Notes
In WB a band can been seen at ~70-85 kDa.
Reviewed Applications
Read 3 reviews rated 4.7 using NBP2-22164 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SLC6A3/DAT1
Long Name
Dopamine Transporter
Alternate Names
DAT1, SLC6A3, VNTR
Gene Symbol
SLC6A3
UniProt
Additional SLC6A3/DAT1 Products
Product Documents for SLC6A3/DAT1 Antibody (mAb16)
Product Specific Notices for SLC6A3/DAT1 Antibody (mAb16)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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