SLC6A3/DAT1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-254
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Golden Syrian Hamster, Hamster, Primate
Cited:
Mouse, Golden Syrian Hamster
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic peptide corresponding to amino acid residues from the intracellular C-terminal region conjugated to KLH. Accession # Q01959
Reactivity Notes
Hamster reported in lierature (PMID: 22357095). Golden Syrian Hamster reactivity reported in scientific literature (PMID: 22357095).
Localization
Integral membrane protein.
Specificity
Specific for endogenous levels of the ~80 kDa DAT protein. Diffuse band may be observed between ~70-85 kDa due to variable glycosylation.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SLC6A3/DAT1 Antibody
![Western Blot: SLC6A3/DAT1 Antibody [NB300-254]](https://resources.bio-techne.com/images/products/SLC6A3-DAT1-Antibody-Western-Blot-NB300-254-img0005.jpg "Western Blot: SLC6A3/DAT1 Antibody [NB300-254]")
Western Blot: SLC6A3/DAT1 Antibody [NB300-254]
Western Blot: SLC6A3/DAT1 Antibody [NB300-254] - Western blot of human caudate lysate showing specific immunolabeling of the ~88k DAT protein.![Western Blot: SLC6A3/DAT1 Antibody [NB300-254]](https://resources.bio-techne.com/images/products/SLC6A3-DAT1-Antibody-Western-Blot-NB300-254-img0004.jpg "Western Blot: SLC6A3/DAT1 Antibody [NB300-254]")
Western Blot: SLC6A3/DAT1 Antibody [NB300-254]
Western Blot: SLC6A3/DAT1 Antibody [NB300-254] - Detection of Dopamine Transporter in human caudate lysate using NB 300-254.Applications for SLC6A3/DAT1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:1000
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:1000
Application Notes
Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 22357095)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SLC6A3/DAT1
Long Name
Dopamine Transporter
Alternate Names
DAT1, SLC6A3, VNTR
Gene Symbol
SLC6A3
UniProt
Additional SLC6A3/DAT1 Products
Product Documents for SLC6A3/DAT1 Antibody
Product Specific Notices for SLC6A3/DAT1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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