SLC6A3/DAT1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-255
Key Product Details
Species Reactivity
Validated:
Human, Primate
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for SLC6A3/DAT1 Antibody
Immunogen
Synthetic peptide corresponding to amino acid residues from the extracellular loop 2 region conjugated to KLH. Accession # Q01959
Reactivity Notes
The antibody has been tested in Western blots of SDS-solubilized human striatal samples and in IHC applications with formaldehyde-fixed human and monkey brain sections.
Specificity
Specific for endogenous levels of the ~80 kDa SLC6A3/DAT1 protein. Diffuse band may be observed between ~70-85 kDa due to variable glycosylation.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SLC6A3/DAT1 Antibody
![Western Blot: SLC6A3/DAT1 Antibody [NB300-255]](https://resources.bio-techne.com/images/products/DAT1-Antibody-Western-Blot-NB300-255-img0001.jpg "Western Blot: SLC6A3/DAT1 Antibody [NB300-255]")
Western Blot: SLC6A3/DAT1 Antibody [NB300-255]
Western Blot: DAT1 Antibody [NB300-255] - Detection of DAT protein in human caudate sample using NB 300-255.![Western Blot: SLC6A3/DAT1 Antibody [NB300-255]](https://resources.bio-techne.com/images/products/DAT1-Antibody-Western-Blot-NB300-255-img0002.jpg "Western Blot: SLC6A3/DAT1 Antibody [NB300-255]")
Western Blot: SLC6A3/DAT1 Antibody [NB300-255]
Western Blot: DAT1 Antibody [NB300-255] - Western blot analysis of DAT1 in SDS-solubilized human caudate using NB300-255 at 1:1000.Applications for SLC6A3/DAT1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:1000
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SLC6A3/DAT1
Long Name
Dopamine Transporter
Alternate Names
DAT1, SLC6A3, VNTR
Gene Symbol
SLC6A3
UniProt
Additional SLC6A3/DAT1 Products
Product Documents for SLC6A3/DAT1 Antibody
Product Specific Notices for SLC6A3/DAT1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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