SLC6A4/5-HTTLPR/Serotonin transporter Antibody (5T7M2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15668
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5T7M2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SLC6A4/5-HTTLPR/Serotonin transporter (NP_001036.1). METTPLNSQKQLSACEDGEDCQENGVLQKVVPTPGDKVESGQISNGYSAVPSPGAGDDTRHSIPATTTTLVAELHQGERETWGKKVDFLLSVIGYAVDLG
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SLC6A4/5-HTTLPR/Serotonin transporter Antibody (5T7M2)
Immunocytochemistry/ Immunofluorescence: SLC6A4/5-HTTLPR/Serotonin transporter Antibody (5T7M2) [SLC6A4/5-HTTLPR/Serotonin transporter] -
Immunocytochemistry/ Immunofluorescence: SLC6A4/5-HTTLPR/Serotonin transporter Antibody (5T7M2) [SLC6A4/5-HTTLPR/Serotonin transporter] - Confocal imaging of paraffin-embedded Human liver cancer tissue using SLC6A4/5-HTTLPR/Serotonin transporter Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody (5T7M2) [SLC6A4/5-HTTLPR/Serotonin transporter] -
Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody (5T7M2) [SLC6A4/5-HTTLPR/Serotonin transporter] - Western blot analysis of rat brain using SLC6A4/5-HTTLPR/Serotonin transporter Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.
Immunoprecipitation: SLC6A4/5-HTTLPR/Serotonin transporter Antibody (5T7M2) [SLC6A4/5-HTTLPR/Serotonin transporter] -
Immunoprecipitation: SLC6A4/5-HTTLPR/Serotonin transporter Antibody (5T7M2) [SLC6A4/5-HTTLPR/Serotonin transporter] - Immunoprecipitation of SLC6A4/5-HTTLPR/Serotonin transporter from 500 ug extracts of HT-29 cells was performed using 2 ug of SLC6A4/5-HTTLPR/Serotonin transporter Rabbit mAb . Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using SLC6A4/5-HTTLPR/Serotonin transporter Rabbit mAb at a dilution of 1:1000.Applications for SLC6A4/5-HTTLPR/Serotonin transporter Antibody (5T7M2)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SLC6A4/5-HTTLPR
Long Name
Solute Carrier Family 6 Member 4
Alternate Names
5-HT Transporter, 5-HTT, 5-HTTLPR, 5HTTLPR, OCD1, Serotonin Transporter 1, SERT
Gene Symbol
SLC6A4
Additional SLC6A4/5-HTTLPR Products
Product Documents for SLC6A4/5-HTTLPR/Serotonin transporter Antibody (5T7M2)
Product Specific Notices for SLC6A4/5-HTTLPR/Serotonin transporter Antibody (5T7M2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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