Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # BU53

Format

BSA Free

Concentration

1 mg/ml

View all SLC7A5/LAT1 Antibodies »

Product Specifications

Immunogen

Plasma cell line 8226

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for SLC7A5/LAT1 Antibody (BU53) - BSA Free

Immunohistochemistry-Paraffin: SLC7A5/LAT1 Antibody (BU53) - BSA Free [NBP2-50465]

Immunohistochemistry-Paraffin: SLC7A5/LAT1 Antibody (BU53) - BSA Free [NBP2-50465]

Immunohistochemistry-Paraffin: SLC7A5/LAT1 Antibody (BU53) [NBP2-50465] - Analysis of a FFPE tissue section of human testis using 1:200 dilution of SLC7A5/LAT1 (BU53) antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
Flow (Cell Surface): SLC7A5/LAT1 Antibody (BU53) - BSA Free [NBP2-50465]

Flow (Cell Surface): SLC7A5/LAT1 Antibody (BU53) - BSA Free [NBP2-50465]

Flow (Cell Surface): SLC7A5/LAT1 Antibody (BU53) [NBP2-50465] - A cell surface stain was performed on Raji cells with SLC7A5 (BU53) antibody NBP2-50465AF647 (blue) along with a matched isotype control NB600-986AF647 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at RT. Both antibodies were conjugated to Alexa Fluor 647.
Flow (Cell Surface): SLC7A5/LAT1 Antibody (BU53) - BSA Free [NBP2-50465]

Flow (Cell Surface): SLC7A5/LAT1 Antibody (BU53) - BSA Free [NBP2-50465]

Flow (Cell Surface): SLC7A5/LAT1 Antibody (BU53) [NBP2-50465] - A cell surface stain was performed on Raji cells with SLC7A5 (BU53) antibody NBP2-50465 (blue) along with a matched isotype control NB600-986 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at RT, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

Applications for SLC7A5/LAT1 Antibody (BU53) - BSA Free

Application
Recommended Usage

Flow (Cell Surface)

1 - 2 ug/ml

Flow Cytometry

1 - 2 ug/ml

Immunohistochemistry

1:100 - 1:300

Immunohistochemistry-Paraffin

1:100 - 1:300
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SLC7A5/LAT1

SLC7A7 is the light subunit of a cationic amino acid transporter that dimerizes with the heavy subunit transporter protein SLC3A2 (CD98). It is expressed on epithelial cell membranes where it transfers cationic and large neutral amino acids from the cell to the extracellular space. Defects in SLC7A7 are a cause of lysinuric protein intolerance.

Long Name

Solute Carrier Family 7 Member 5

Alternate Names

4F2LC, CD98lc, E16, LAT1, MPE16, TA1

Gene Symbol

SLC7A5

Additional SLC7A5/LAT1 Products

Product Documents for SLC7A5/LAT1 Antibody (BU53) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SLC7A5/LAT1 Antibody (BU53) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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