SLMAP Antibody

Immunocytochemistry/ Immunofluorescence: SLMAP Antibody [NBP1-81397]

Immunocytochemistry/Immunofluorescence: SLMAP Antibody [NBP1-81397] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397]

Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397] - Staining of human smooth muscle shows high expression.

Western Blot: SLMAP Antibody [NBP1-81397]

Western Blot: SLMAP Antibody [NBP1-81397] - Analysis in human cell line CAPAN-2.

Western Blot: SLMAP Antibody [NBP1-81397]

Western Blot: SLMAP Antibody [NBP1-81397] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397]

Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397] - Staining of human testis.

Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397]

Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397] - Staining of human lymph node.

Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397]

Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397] - Staining of human liver using Anti-SLMAP antibody NBP1-81397.

Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397]

Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397] - Staining of human smooth muscle using Anti-SLMAP antibody NBP1-81397.

Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397]

Immunohistochemistry-Paraffin: SLMAP Antibody [NBP1-81397] - Staining of human liver shows low expression as expected.

Western Blot: SLMAP Antibody [NBP1-81397] -

Western Blot: SLMAP Antibody [NBP1-81397] - Postnatal expression of SLMAP3 in Tg mice.(A) Schematic representation of the SLMAP3 transgene construct comprising the forkhead associated domain (FHA), the leucine zipper coiled-coil region (LZ), & the transmembrane domain 2 (TM2) in frame with 6-myc tag, driven by alpha-MHC promoter. (B) Number of SLMAP3 transgene copies in hearts. DNA was isolated from hearts of mice & qRT-PCR was performed with appropriate primers to assess copies of Myc-SLMAP3 transgene. (C) SLMAP3 transgene protein expression in Tg mice. Western blot of heart lysate from Wt & Tg mice with anti-myc in high (H), moderate (M), & low (L) SLMAP3-six myc expression, GAPDH was used as loading control. (D) Endogenous SLMAP isoforms in Wt & Tg myocardium. Western blots of SLMAP isoforms in Wt & Tg mice at 5 weeks of age with anti-SLMAP (~35 kDa, ~43 kDa, ~81 kDa, ~91 kDa, & 110 kDa) & anti-myc antibodies (~110 kDa). SLMAP3 image was acquired with a longer exposure of the same membrane. (E) Quantification & fold change of protein expression levels of SLMAP1 (~35 kDa) & SLMAP2 (~43 kDa) isoforms in Tg mice compared to Wt age-matched littermates at 5 weeks of age; Wt corresponds to 1 (100%), n = 3. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30934005), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: SLMAP Antibody [NBP1-81397] -

Western Blot: SLMAP Antibody [NBP1-81397] - Expression of calcium handling proteins & their phosphorylation in microsomes.(A) Western blot analysis of SERCA2a protein expression in microsomal fractions & total protein acquired by stain-free. (B) Quantification of SERCA2a in microsomal fractions normalized to total protein assessed by stain free technology in Wt (n = 4) & Tg (n = 7) mice. (C) Western blots for total ryanodine receptor 2 (t-RyR2), it’s phosphorylation on serine 2808 (p-RyR2 ser 2808), calsequestrin (CSQ), total phospholamban (t-PLN) & it’s phosphorylation on serine 16 (p-PLN ser16) in microsomal fractions from 5 weeks old Tg & Wt mice hearts. Anti-calreticulin was used as loading control. Tg SLMAP3 was detected by anti-myc (Myc-SLMAP3). (D) Quantification of RyR2, CSQ, & PLN (monomeric) protein expression in Wt & Tg mice. *p<0.05, #p = 0.053, n (Wt) = 5, n (Tg) = 4. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30934005), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: SLMAP Antibody [NBP1-81397] -

Western Blot: SLMAP Antibody [NBP1-81397] - Expression of calcium handling proteins & their phosphorylation in microsomes.(A) Western blot analysis of SERCA2a protein expression in microsomal fractions & total protein acquired by stain-free. (B) Quantification of SERCA2a in microsomal fractions normalized to total protein assessed by stain free technology in Wt (n = 4) & Tg (n = 7) mice. (C) Western blots for total ryanodine receptor 2 (t-RyR2), it’s phosphorylation on serine 2808 (p-RyR2 ser 2808), calsequestrin (CSQ), total phospholamban (t-PLN) & it’s phosphorylation on serine 16 (p-PLN ser16) in microsomal fractions from 5 weeks old Tg & Wt mice hearts. Anti-calreticulin was used as loading control. Tg SLMAP3 was detected by anti-myc (Myc-SLMAP3). (D) Quantification of RyR2, CSQ, & PLN (monomeric) protein expression in Wt & Tg mice. *p<0.05, #p = 0.053, n (Wt) = 5, n (Tg) = 4. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30934005), licensed under a CC-BY license. Not internally tested by Novus Biologicals.