Key Product Details

Species Reactivity

Validated:

Human, Mouse, Porcine

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # SLP-76/03

Format

BSA Free

Concentration

1.0 mg/ml

View all SLP-76/LCP2 Antibodies »

Product Specifications

Immunogen

Bacterially expressed fusion protein representing amino acids 216-434 of human SLP76 with histidine tag

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Specificity

The polyclonal antibody reacts with SLP76, a 76kDa cytosolic adaptor protein that is involved in signaling of various hematopoietic cells, such as T cells, mast cells or neutrophils; in B cells, however, it is replaced by SLP65.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SLP-76/LCP2 Antibody (SLP-76/03) - BSA Free

Western Blot: SLP-76/LCP2 Antibody (SLP-76/03)BSA Free [NB500-537]

Western Blot: SLP-76/LCP2 Antibody (SLP-76/03)BSA Free [NB500-537]

Western Blot: SLP-76/LCP2 Antibody (SLP-76/03) [NB500-537] - Lane 1: HPB-ALL human peripheral blood T cell leukemia cell line Lane 2: JURKAT human peripheral blood T cell leukemia cell line Lane 3: RAMOS human Burkitt lymphoma cell line

Applications for SLP-76/LCP2 Antibody (SLP-76/03) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1:1000
Application Notes
Predicted molecular weight: 64 kDa.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SLP-76/LCP2

SLP76 (SH2 domain-containing leukocyte protein of 76 kDa) is a cytosolic adaptor protein which translocates to the plasma mambrane and is involved in multiple signaling pathways in T cells, mast cells, neutrophils and platelets; B cells express its analog SLP65/BLNK (B cell linker protein). SLP76 is phosphorylated by Syk-family and Tec-family tyrosine kinases and couples them to the phosphorylation and activation of PLC-gamma. Via Gads or Grb2, SLP76 also associates with LAT adaptor by involvement of SLP76 proline-rich region. The SH2 domain of SLP76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk-mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation.

Long Name

SH2 Domain-containing Leukocyte Protein of 76 kDa

Alternate Names

LCP2, SLP76

Entrez Gene IDs

16822 (Mouse)

Gene Symbol

LCP2

Additional SLP-76/LCP2 Products

Product Documents for SLP-76/LCP2 Antibody (SLP-76/03) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SLP-76/LCP2 Antibody (SLP-76/03) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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