SLP-76/LCP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1439

Novus Biologicals, part of Bio-Techne
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NB100-1439
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all SLP-76/LCP2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence ALRNVPFRSEV-C corresponding to N-Terminus according to NP_005556.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SLP-76/LCP2 Antibody

Western Blot: SLP-76/LCP2 Antibody [NB100-1439]

Western Blot: SLP-76/LCP2 Antibody [NB100-1439]

Western Blot: SLP-76/LCP2 Antibody [NB100-1439] - (0.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for SLP-76/LCP2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 80 kDa band observed in Jurkat lysates is reported in scientific literature (PMID: 7706237).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SLP-76/LCP2

SLP76 (SH2 domain containing leukocyte protein of 76 kDa) is a hematopoietic cell-specific adaptor protein that is crucial for T-cell receptor (TCR) signaling, hemostasis and platelet function. TCR ligation and fibrinogen binding to integrin alpha 2 beta 3 stimulates the phosphorylation of the tyrosine residues in the amino terminus, and facilitates SLP76 binding to the SH2 domain of Vav, which can activate JNK. SLP76 also comprises a proline-rich domain region that associates with the SH3 domain of Grb2 linking SLP76 to the Ras to Raf to ERK1 & 2 signaling pathway, LAT, PLC gamma, Fyn-binding protein (SLAP130), the SH2 containing phosphatase 1 and Nck, which mediates the regulation of cytoskeletal actin polymerization. Phosphorylation of tyrosine 145 has been shown to be important for optimal SLP76 function.

Long Name

SH2 Domain-containing Leukocyte Protein of 76 kDa

Alternate Names

LCP2, SLP76

Entrez Gene IDs

3937 (Human)

Gene Symbol

LCP2

UniProt

NP_005556. (Human)

Additional SLP-76/LCP2 Products

Product Documents for SLP-76/LCP2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SLP-76/LCP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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