SLP-76/LCP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87033

Novus Biologicals, part of Bio-Techne
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NBP1-87033
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SLP-76/LCP2 Antibodies »

Product Summary for SLP-76/LCP2 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GARFLNLTENDIQKFPKLRVPILSKLSQEINKNEERRSIFTRKPQVPRFPEETESHEEDNGGWSSFEEDDYESPNDDQDGE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SLP-76/LCP2 Antibody

Western Blot: SLP-76/LCP2 Antibody [NBP1-87033]

Western Blot: SLP-76/LCP2 Antibody [NBP1-87033]

Western Blot: SLP-76/LCP2 Antibody [NBP1-87033] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: HEL
Immunohistochemistry-Paraffin: SLP-76/LCP2 Antibody [NBP1-87033]

Immunohistochemistry-Paraffin: SLP-76/LCP2 Antibody [NBP1-87033]

Immunohistochemistry-Paraffin: SLP-76/LCP2 Antibody [NBP1-87033] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry: SLP-76/LCP2 Antibody [NBP1-87033]

Immunohistochemistry: SLP-76/LCP2 Antibody [NBP1-87033]

Immunohistochemistry: SLP-76/LCP2 Antibody [NBP1-87033] - Staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.
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Applications for SLP-76/LCP2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SLP-76/LCP2

SLP76 (SH2 domain containing leukocyte protein of 76 kDa) is a hematopoietic cell-specific adaptor protein that is crucial for T-cell receptor (TCR) signaling, hemostasis and platelet function. TCR ligation and fibrinogen binding to integrin alpha 2 beta 3 stimulates the phosphorylation of the tyrosine residues in the amino terminus, and facilitates SLP76 binding to the SH2 domain of Vav, which can activate JNK. SLP76 also comprises a proline-rich domain region that associates with the SH3 domain of Grb2 linking SLP76 to the Ras to Raf to ERK1 & 2 signaling pathway, LAT, PLC gamma, Fyn-binding protein (SLAP130), the SH2 containing phosphatase 1 and Nck, which mediates the regulation of cytoskeletal actin polymerization. Phosphorylation of tyrosine 145 has been shown to be important for optimal SLP76 function.

Long Name

SH2 Domain-containing Leukocyte Protein of 76 kDa

Alternate Names

LCP2, SLP76

Gene Symbol

LCP2

Additional SLP-76/LCP2 Products

Product Documents for SLP-76/LCP2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SLP-76/LCP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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