Slug Antibody (3C12)

Novus Biologicals, part of Bio-Techne | Catalog # H00006591-M05

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 3C12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Slug Antibodies »

Product Specifications

Immunogen

SNAI2 (NP_003059, 97 a.a. ~ 169 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KDHSGSESPISDEEERLQSKLSDPHAIEAEKFQCNLCNKTYSTFSGLAKHKQLHCDAQSRKSFSCKYCDKEYV

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

SNAI2 (3C12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Slug Antibody (3C12)

Western Blot: Slug Antibody (3C12) [H00006591-M05]

Western Blot: Slug Antibody (3C12) [H00006591-M05]

Western Blot: Slug Antibody (3C12) [H00006591-M05] - Analysis of SNAI2 expression in NIH/3T3 (Cat # L018V1).
Immunocytochemistry/ Immunofluorescence: Slug Antibody (3C12) [H00006591-M05]

Immunocytochemistry/ Immunofluorescence: Slug Antibody (3C12) [H00006591-M05]

Immunocytochemistry/Immunofluorescence: Slug Antibody (3C12) [H00006591-M05] - Analysis of monoclonal antibody to SNAI2 on U-2 OS cell. Antibody concentration 10 ug/ml
Western Blot: Slug Antibody (3C12) [H00006591-M05]

Western Blot: Slug Antibody (3C12) [H00006591-M05]

Western Blot: Slug Antibody (3C12) [H00006591-M05] - Analysis of SNAI2 expression in PC-12 (Cat # L012V1).
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Applications for Slug Antibody (3C12)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate, tissue lysate and recombinant protein for WB. It has been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Slug

This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects.

Alternate Names

SLUGH1, SNAI2, SNAIL2, WS2D

Entrez Gene IDs

6591 (Human)

Gene Symbol

SNAI2

OMIM

602150 (Human)

UniProt

NP_003059 (Human)

Additional Slug Products

Product Documents for Slug Antibody (3C12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Slug Antibody (3C12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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