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Slug Antibody (BLR139H)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14719

Recombinant Monoclonal
Novus Biologicals, part of Bio-Techne
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NBP3-14719

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR139H

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Slug Antibody (BLR139H)

Immunogen

between 75 and 125

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for Slug Antibody (BLR139H)

Immunoprecipitation: Slug Antibody (BLR139H) [NBP3-14719]

Immunoprecipitation: Slug Antibody (BLR139H) [NBP3-14719]

Immunoprecipitation: Slug Antibody [NBP3-14719] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from MDA-MB-435 cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiSlug recombinant monoclonal antibody used for IP at 20 ul/mg lysate. Slug wasalso immunoprecipitated by rabbit anti-Slug antibody. For blotting immunoprecipitated SLUG,was used at 1:1000. Chemiluminescence withan exposure time of 30 seconds.
Slug Antibody (BLR139H)

Western Blot-Slug Antibody (BLR139H)-NBP3-14719-

Western Blot-Slug Antibody (BLR139H)-NBP3-14719-Whole cell lysate (50 ug) from MCF-7, HeLa, HEK293T, MDAMB-435, Jurkat, MDA-MB-231, Hep-G2, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit antiSlug recombinant monoclonal antibody [BLR139H] used at 1:1000. Secondary: HRP conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Applications for Slug Antibody (BLR139H)

Application
Recommended Usage

Immunoprecipitation

20 ul/1 mg lysate

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: Slug

SLUG encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects.

Alternate Names

SLUGH1, SNAI2, SNAIL2, WS2D

Gene Symbol

SNAI2

Additional Slug Products

Product Documents for Slug Antibody (BLR139H)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Slug Antibody (BLR139H)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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