Skip to main content

Slug Antibody (OTI1A6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03886

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP2-03886

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A6

Concentration

1 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human SNAI2 (NP_003059) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Slug Antibody (OTI1A6)

Western Blot: Slug Antibody (OTI1A6) [NBP2-03886]

Western Blot: Slug Antibody (OTI1A6) [NBP2-03886]

Western Blot: SLUG Antibody (1A6) [NBP2-03886] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLUG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLUG.
Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]

Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]

Immunohistochemistry-Paraffin: Slug Antibody (1A6) [NBP2-03886] - Mouse glioma stained with Slug antibody. Image from verified customer review.
Flow Cytometry: Slug Antibody (OTI1A6) [NBP2-03886]

Flow Cytometry: Slug Antibody (OTI1A6) [NBP2-03886]

Flow Cytometry: SLUG Antibody (1A6) [NBP2-03886] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SLUG antibody, and then analyzed by flow cytometry.

Applications for Slug Antibody (OTI1A6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:1000
Application Notes
For IHC-P, we recommend using HIER with Tris-EDTA, pH8.0.

Reviewed Applications

Read 1 review rated 5 using NBP2-03886 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Slug

This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq].

Alternate Names

SLUGH1, SNAI2, SNAIL2, WS2D

Entrez Gene IDs

6591 (Human)

Gene Symbol

SNAI2

Additional Slug Products

Product Documents for Slug Antibody (OTI1A6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Slug Antibody (OTI1A6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...
Loading...