Slug Antibody (OTI1A6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03886
![Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]](https://resources.bio-techne.com/images/products/SLUG-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-03886-img0003.jpg "Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]")
![Western Blot: Slug Antibody (OTI1A6) [NBP2-03886]](https://resources.bio-techne.com/images/products/SLUG-Antibody-1A6-Western-Blot-NBP2-03886-img0002.jpg "Western Blot: Slug Antibody (OTI1A6) [NBP2-03886]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1A6
Concentration
1 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human SNAI2 (NP_003059) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Slug Antibody (OTI1A6)
Western Blot: Slug Antibody (OTI1A6) [NBP2-03886]
Western Blot: SLUG Antibody (1A6) [NBP2-03886] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLUG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLUG.![Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]](https://resources.bio-techne.com/images/products/Slug-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-03886-img0009.jpg "Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]")
Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]
Immunohistochemistry-Paraffin: Slug Antibody (1A6) [NBP2-03886] - Mouse glioma stained with Slug antibody. Image from verified customer review.![Flow Cytometry: Slug Antibody (OTI1A6) [NBP2-03886]](https://resources.bio-techne.com/images/products/SLUG-Antibody-1A6-Flow-Cytometry-NBP2-03886-img0001.jpg "Flow Cytometry: Slug Antibody (OTI1A6) [NBP2-03886]")
Flow Cytometry: Slug Antibody (OTI1A6) [NBP2-03886]
Flow Cytometry: SLUG Antibody (1A6) [NBP2-03886] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SLUG antibody, and then analyzed by flow cytometry.![Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]](https://resources.bio-techne.com/images/products/SLUG-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-03886-img0004.jpg "Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]")
![Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]](https://resources.bio-techne.com/images/products/SLUG-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-03886-img0005.jpg "Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]")
![Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]](https://resources.bio-techne.com/images/products/SLUG-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-03886-img0006.jpg "Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]")
![Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]](https://resources.bio-techne.com/images/products/SLUG-Antibody-1A6-Immunohistochemistry-Paraffin-NBP2-03886-img0007.jpg "Immunohistochemistry-Paraffin: Slug Antibody (OTI1A6) [NBP2-03886]")
Applications for Slug Antibody (OTI1A6)
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:1000
Application Notes
For IHC-P, we recommend using HIER with Tris-EDTA, pH8.0.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NBP2-03886 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Slug
Additional Slug Products
Product Documents for Slug Antibody (OTI1A6)
Product Specific Notices for Slug Antibody (OTI1A6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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