SMAC/Diablo Antibody (78-1-118)

Novus Biologicals, part of Bio-Techne | Catalog # NB500-213

Novus Biologicals, part of Bio-Techne
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NB500-213
NB500-213SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 78-1-118

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all SMAC/Diablo Antibodies »

Product Specifications

Immunogen

Recombinant mature human Smac (lacks amino acids 1-55 present in Smac precursor). [UniProt# Q9NR28]

Localization

Mitochondrial but released into the cytosol when cells undergo apoptosis.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SMAC/Diablo Antibody (78-1-118)

Western Blot: SMAC/Diablo Antibody (78-1-118) [NB500-213]

Western Blot: SMAC/Diablo Antibody (78-1-118) [NB500-213]

Western Blot: Smac Antibody (78-1-118) [NB500-213] - HeLa whole cell extract lysates.

Applications for SMAC/Diablo Antibody (78-1-118)

Application
Recommended Usage

Immunoprecipitation

1:10 - 1:500

Western Blot

1:500
Application Notes
This Smac (78-1-118) antibody is useful for Immunoprecipitation and Western blot, where a band is seen at ~25 kDa. For better results, IP prior to Western blot is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4.8 using NB500-213 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SMAC/Diablo

Smac/DIABLO, a mitochondrial protein, activates various forms of apoptosis. This activation may be due to the neutralization of one or more members of the IAP family of apoptosis inhibitory proteins. Smac exits the mitochondria and enters the cytosol during certain apoptosis triggered events. Mitochondrial-mediated apoptosis is important in animal development and tissue homeostasis, with alterations resulting in a range of malignant disorders. Upon apoptotic stimuli, the mitochondrial proteins cytochrome c and Smac/DIABLO are released into the cytosol. The release of these proteins, however, occurs via different mechanisms (2,3).

Long Name

Second Mitochondria-derived Activator of Caspase

Alternate Names

Diablo

Entrez Gene IDs

56616 (Human); 66593 (Mouse); 288753 (Rat)

Gene Symbol

DIABLO

UniProt

Q9NR28

Additional SMAC/Diablo Products

Product Documents for SMAC/Diablo Antibody (78-1-118)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SMAC/Diablo Antibody (78-1-118)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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