SMAC/Diablo Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56311

Novus Biologicals, part of Bio-Techne
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NB100-56311
NB100-56311SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all SMAC/Diablo Antibodies »

Product Specifications

Immunogen

This rabbit polyclonal antibody was developed against two synthetic peptides corresponding to amino acids 199 to 212 and amino acids 226 to 239 of human Smac (Genbank accession no. AAF87716).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22751125).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMAC/Diablo Antibody

Western Blot: SMAC/Diablo Antibody [NB100-56311]

Western Blot: SMAC/Diablo Antibody [NB100-56311]

Western Blot: SMAC/Diablo Antibody [NB100-56311] - Analysis of Smac (DIABLO) in HeLa lysate using Smac antibody at 2 ug/ml.
Immunohistochemistry: SMAC/Diablo Antibody [NB100-56311]

Immunohistochemistry: SMAC/Diablo Antibody [NB100-56311]

Immunohistochemistry: SMAC/Diablo Antibody [NB100-56311] - Analysis of human prostate using Smac antibody at 10 ug/ml.

Applications for SMAC/Diablo Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500. Use reported in scientific literature (Hu et al (2004))

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

0.5-2 ug/ml
Application Notes
Use in Immunohistochemistry-Paraffin reported in scientific literature (Ren et al. (2006))

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMAC/Diablo

Recently, three different laboratories have identified a molecule, Smac/DIABLO, which binds with inhibitor of apoptosis proteins (IAPs) and neutralizes IP inhibition of effector caspases. Multiple IAP molecules, including c-IAP1, c-IAP2, XIAP, and survivin are able to bind Smac. Smac/DIABLO is a mitochondrial protein, which is released into cytosol during apoptosis. The function of Smac is similar to that of Drosophila apoptosis activating proteins Reaper, Hid and Grim, which have been proposed to function by neutralizing the activity of IAPs. Smac promotes caspase-3 and caspase-9 activation by removing inhibitors rather than directly acting on Apaf-1, procaspases, and cytochrome c. The full-length cDNA of Smac encodes an open reading frame of 239 amino acids, predicting a protein with a molecular weight of 27 kDa. However, the mature protein purified from HeLa cells is only about 25 kDa.

Long Name

Second Mitochondria-derived Activator of Caspase

Alternate Names

Diablo

Gene Symbol

DIABLO

UniProt

Q9NR28

Additional SMAC/Diablo Products

Product Documents for SMAC/Diablo Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SMAC/Diablo Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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