SMAD1/5/8/9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56656

Novus Biologicals, part of Bio-Techne
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NB100-56656
NB100-56656SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SMAD1/5/8/9 Antibodies »

Product Specifications

Immunogen

This antibody was developed against two synthetic peptides corresponding to amino acids 19-33 (GWKQGDEEEKWAEKA) and amino acids 315-329 (SNVNRNSTIENTRRH) of human SMAD1.

Specificity

The synthetic peptide immunogens used to make this antibody are 100% conserved between SMAD1/5/8/9. Therefore, the antibody recognizes SMADs 1, 5, 8, and 9. The antibody may cross-react with SMAD2 or 3. The actual SMADs detected with this antibody may vary according to cell or tissue-type specific expression.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMAD1/5/8/9 Antibody

Western Blot: SMAD1/5/8/9 Antibody [NB100-56656]

Western Blot: SMAD1/5/8/9 Antibody [NB100-56656]

Western Blot: SMAD1/5/8/9 Antibody [NB100-56656] - Analysis using SMAD1/5/8/9 antibody. Lysate from Saos-2 cells probed with SMAD antibody.

Applications for SMAD1/5/8/9 Antibody

Application
Recommended Usage

Western Blot

1 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMAD1/5/8/9

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them.

Alternate Names

BSP1, BSP-1, hSMAD1, JV4-1SMAD, mothers against DPP homolog 1 (Drosophila), MAD homolog 1, MADH1JV41, MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila), Mad-related protein 1, mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, SMAD family member 1SMAD 1, SMAD, mothers against DPP homolog 1, Smad1, TGF-beta signaling protein 1, transforming growth factor-beta signaling protein 1

Entrez Gene IDs

17125 (Mouse); 25671 (Rat)

Gene Symbol

SMAD1

UniProt

Q15797

Additional SMAD1/5/8/9 Products

Product Documents for SMAD1/5/8/9 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SMAD1/5/8/9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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