SMAD1/5/8/9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-962

Novus Biologicals, part of Bio-Techne
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NB600-962SS
NB600-962
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SMAD1/5/8/9 Antibodies »

Product Specifications

Immunogen

This antibody was raised against two synthetic peptides corresponding to amino acids 18-32 (LGWKQGDEEEKWAEK) and amino acids 315-329 (SNVNRNSTIENTRRH) of human SMAD1. GenBank Accession No. AAP36050.1. They are 100% conserved or highly conserved between human and multiple species.

Reactivity Notes

Predicted to react with Chicken, Chimpanzee, Invertebrates, Mammals, Porcine, Xenopus and Zebrafish.

Localization

Cytoplasmic and Nuclear

Specificity

The amino acid sequences used to make this antibody are 100% conserved between SMAD1/5/8/9. Therefore, the antibody recognizes SMADs 1, 5, 8, and 9. The antibody may cross-react with SMAD2 or 3. The actual SMADs detected with this antibody may vary according to cell or tissue-type specific expression.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMAD1/5/8/9 Antibody

Western Blot: SMAD1/5/8/9 Antibody [NB600-962]

Western Blot: SMAD1/5/8/9 Antibody [NB600-962]

Western Blot: SMAD1/5/8/9 Antibody [NB600-962] - Analysis using SMAD antibody. Lysate from A549 cells probed with antibody at 0.5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Applications for SMAD1/5/8/9 Antibody

Application
Recommended Usage

Western Blot

0.5 - 2 ug/ml
Application Notes
Western Blot: Detects a band of approximately 52 kDa.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMAD1/5/8/9

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.

Alternate Names

BSP1, BSP-1, hSMAD1, JV4-1SMAD, mothers against DPP homolog 1 (Drosophila), MAD homolog 1, MADH1JV41, MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila), Mad-related protein 1, mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, SMAD family member 1SMAD 1, SMAD, mothers against DPP homolog 1, Smad1, TGF-beta signaling protein 1, transforming growth factor-beta signaling protein 1

Entrez Gene IDs

17129 (Mouse); 59328 (Rat)

Gene Symbol

SMAD1

Additional SMAD1/5/8/9 Products

Product Documents for SMAD1/5/8/9 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SMAD1/5/8/9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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